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Western blot analysis of native (lanes 1-3) and denatured (lanes 4-6) cell lysates from human A431 (lane 1 & 4), A549 (lane 2 & 5), and MDA-MB-231 (lane 3 & 6). The blots were probed with mouse monoclonal anti-Integrin β1 (IM0061) at 1:1000 dilution.