ECM Logo Bar
Search :
Technical Data Sheet

AK6970

image
image
image
Argonaute 2 Phospho-Regulation
Antibody Sampler Kit
Price

$395

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
Kit Summary
The argonaute 2 (Ago2) phospho-regulation antibody sampler kit can be used to detect phosphorylation of Ser-389 and Tyr-393 relative to total Ago2 expression levels. The kit includes rabbit polyclonal phospho-specific antibodies to Ser-389 and Tyr-393, as well as rat monoclonal and rabbit polyclonal antibodies for detection of total Ago2. The kit also includes anti-Rabbit Light Chain specific:HRP and anti-Rat Ig:HRP secondary reagents for detection of antibodies in western blot, ELISA, or immunocytochemistry.
image
image
Enlarge

Western blot analysis of mouse recombinant Ago2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2 (AP5281) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Ser-387) phospho-specific antibody (lanes 3 & 4).

image
Enlarge

Western blot analysis of human A431 cells treated with EGF (100 ng/ml for 60 min.) (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rat monoclonal anti-Ago2 (AM5271) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Tyr-393) phospho-specific antibody (lanes 3 & 4).

image
Background
Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response. These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. Among the most important components in these complexes are argonaute proteins. There are four members in the mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFR phosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylates Ago2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression.

Background References
Peters, L. & Meister, G. (2007) Mol Cell 26: 611.
Filipowicz, W. et al. (2008) Nat Rev Genet. 9:102.
Shen, J. et al. (2013) Nature 497:383.
Horman, S.R. et al. (2013) Mol Cell 50:356.
Horman, S.R. et al. (2013) Mol Cell 50:356.
Buffer and Storage
Primary antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. BP4971 is supplied in phosphate-buffered saline and 0.09% sodium azide. Store all at –20°C. Stable for 1 year.
imageRelated Products
AK6340
Akt Phospho-Regulation Antibody Sampler Kit

AK6970
Argonaute 2 Phospho-Regulation Antibody Sampler Kit

EK6160
EGFR Phospho-Regulation Antibody Sampler Kit

MS3001
Anti-Mouse Ig:HRP Donkey Polyclonal

MS3011
Anti-Mouse Ig:DyLight® 488 Goat Polyclonal

RS3101
Anti-Rat IgG:HRP Donkey Polyclonal

RS3111
Anti-Rat IgG Light Chain:DyLight® 594 Goat Polyclonal

RS3201
Anti-Rabbit Ig:HRP Goat Polyclonal

RS3241
Anti-Rabbit Ig Light-Chain Specific, unconjugated Mouse Monoclonal

RS3261
Anti-Rabbit Ig:DyLight® 488 Goat Polyclonal

RS3271
Anti-Rabbit Ig:DyLight® 594 Goat Polyclonal

New Releases
PM4991
image
PKC (α,β,γ) M499
Mouse Monoclonal
image
TP5671
image
TRPM7 (Ser-1513), phospho-specific
Rabbit Polyclonal
image
CM0331
image
CD55 (Extracellular region) M033
Mouse Monoclonal
image