AL8011

Actin (Rabbit Skeletal Muscle)

Lysate

Price

Size

$75

100 μl

Application

Dilution

10 μl/lane

End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.

Background

Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four α isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (β and γ). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin.

For preparation of actin lysate, purified rabbit skeletal muscle actin was quantified using the BCA method (Pierce) before diluting to the final concentration in gel electrophoresis sample buffer.

Buffer and Storage

Actin Lysate is supplied at a concentration of 20 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.

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