ECM Logo Bar
Search :
Technical Data Sheet

AL9501

image
image
image
A431 + Pervanadate
Lysate
Price
Size

$75
100 μl

image
image
Enlarge

Western blot analysis of A431, HeLa, and Jurkat cells (20 µg/lane) untreated or treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-Phosphotyrosine rabbit polyclonal.

Application
Dilution


WB
20 μl/lane



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. When cells are treated with pervanadate for 30 minutes they undergo significant tyrosine phosphorylation, as shown by western blotting using anti-Phosphotyrosine.

Confluent cultures of A431 cells were serum starved overnight. Cells were then either left untreated (Cat.# AL9401) or treated with pervanadate (1 mM) for 30 minutes at 37°C (cat.# AL9501). Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

Buffer and Storage
Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
imageRelated Products
AL9001
A431 Calyculin A Control Lysate

AL9101
A431 + Calyculin A (30min) Lysate

AL9201
A431 EGF Control Lysate

AL9301
A431 + EGF (5 min) Lysate

AL9401
A431 Pervanadate Control Lysate

New Releases
IM0041
image
Integrin β1 (Extracellular region) M004
Mouse Monoclonal
image
IK6750
image
Integrin α family
Antibody Sampler Kit
image
IM5941
image
Integrin αL (Extracellular region) M594
Mouse Monoclonal
image