ECM Logo Bar
Search :
Technical Data Sheet

AM0091

image
image
image
Annexin A2 M009
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu
36 kDa

image
image
Enlarge

Western blot of human A431 (lane 1), MDA-MB-231 (lane 2), LNCaP (lane 3), MeWo (lane 4), HUVEC (lane 5), Jurkat (lane 6), K562 (lane 7), and PC3 (lane 8) cell lysates. The blot was probed with mouse monoclonal anti-Annexin A2 antibody (AM0091) at 1:500 (lanes 1-8).

image
Enlarge

Immunocytochemical labeling of Annexin A2 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Annexin A2 (clone M009). The antibody was detected using goat anti-mouse DyLight® 594.

image
Application
Dilution


ELISA
1:1000


ICC
1:100


IP-MS
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin α1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family has been implicated as regulators of such diverse processes as ion-flux, endocytosis and exocytosis, and cellular adhesion. Annexin A2 (calpactin I, chromobindin 8, p36, Lipocortin II, PAP-IV, or Protein I) is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of cortocosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.


Background References
Feinberg, J.M. et al. (1991) J Histochem & Cytochem. 39:955.
Grewal, T. & Enrich, C. (2006) Bioessays 28(12):1211.
Immunogen
Clone M009 was generated from a proprietary antigen related to human Annexin A2.
Buffer and Storage
Mouse monoclonal, protein G purified antibody is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Specificity
Clone M009 detects a 36 kDa* protein corresponding to the molecular mass of Annexin A2 on SDS-PAGE immunoblots of human A431, MDA-MB-231, MeWo, HUVEC, and PC3 cells. The antibody also works for ELISA, immunocytochemical labeling, and immunoprecipitation applications. In addition, mass spectrometry analysis of immunoprecipitates using AM0091 in human A431 cell lysates confirms that this antibody only detects Annexin A2 protein.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
AK6060
Actin & Tubulin Antibody Sampler Kit

AM2981
Annexin A2 (C-terminal region) Mouse Monoclonal

AM3301
Annexin A6 Mouse Monoclonal

OK7670
Organelle Marker Immunocytochemistry Kit

RM3351
RasGAP Mouse Monoclonal

New Releases
GP5781
image
Girdin (Ser-1674), phospho-specific
Rabbit Polyclonal
image
IK6700
image
Integrin β family
Antibody Sampler Kit
image
IM5821
image
Integrin β1 (Extracellular region) M582
Mouse Monoclonal
image