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Technical Data Sheet

AM2951

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β-Adaptin (N-terminal region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
106 kDa

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Western blot of human Jurkat cell lysate. The blot was probed with mouse monoclonal anti-β-Adaptin (N-terminal region) antibody at 1:500 (lane 1) and 1:2000 (lane 2).

Application
Dilution


ELISA
1:2000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Adaptins are essential for the formation of adaptor complexes of clathrin-coded vesicles. Adaptins interact with the cytoplasmic domains of membrane-spanning receptors in the course of their endocytic/exocytic transport. They represent 1 of at least 4 components of intracellular transport and receptor downregulation pathways, the others being α/β/γ-adaptins, receptors, and ligand molecules. β-adaptins have a bipartite structure with invariant N-terminal and variable C-terminal domains. The former interact with the uniform component of coded vesicles, the clathrin lattice. In contrast, the C-terminal domain provides a set of recognition sequences for other proteins and therefore functions as part of a selector domain responsible for the specific entrapment of membrane-bound proteins in vesicles.


Background References
Sosa, M.A. (1996) Biocell. 20(3):301.
Boehm M. & Bonifacino J.S. (2002) Gene. 286(2):175.
Immunogen
Clone (M295) was generated from a sequence corresponding to amino acids near the N-terminal region of the human β1 subunit of the adaptor related protein complex 1. This sequence has high homology to rat and mouse β1-Adaptin.
Buffer and Storage
Mouse monoclonal, protein G purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Specificity
This antibody detects a 106 kDa* protein corresponding to the molecular mass of β-Adaptin on SDS-PAGE immunoblots of human Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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