ECM Logo Bar
Search :
Technical Data Sheet

AM3341

image
image
image
Abp1 (C-terminal region)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu
55 kDa

image
image
Enlarge

Western blot of Jurkat (lane 1), HeLa (lane 2), mouse brain (lane 3), and A431 (lane 4). The blot was probed with mouse monoclonal anti-Abp1 (C-terminal region) antibody at 1:500.

image
Enlarge

Immunocytochemical labeling of Abp1 in aldehyde-fixed and NP-40-permeabilized differentiated A431 cells. The cells were labeled with mouse monoclonal anti-Abp1 (AM3341) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.

image
Application
Dilution


ELISA
1:2000


ICC
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20 Arc, and p16-Arc. Several actin binding proteins, WASP, cortactin, and Abp1, regulate the activity of the Arp2/3 complex. Abp1 (SH3P7, HIP-55) is the mammalian homologue of the yeast Abp1p, which has been shown to bind actin and to have roles in endocytosis. Abp1 contains a C-terminal Src homology 3 domain and an N-terminal actin depolymerization factor (ADF-H/C) domain that is homologous to a similar domain found in the actin severing protein, cofilin. Abp1 can bind Src kinases, hematopoietic progenitor kinase 1 (HPK-1), myosin I heavy chain kinase, and dynamin. Thus, Abp1 may be important for regulation of the actin cytoskeleton through its ability to interact with F-actin and various other cytoskeletal and cell signaling proteins.


Background References
Larbolette, O. et al. (1999) Mol Cell Biol. 19(2):1539.
Kessels, M.M. et al. (2001) J Cell Biol. 153(2):351.
Olazabal, I.M. & Machesky, L.M. (2001) J Cell Biol. 154(4):679.
Immunogen
Clone (M334) was generated from a recombinant sequence that included amino acids in the C-terminal region of human Abp1.
Buffer and Storage
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Specificity
This antibody detects a 55 kDa* protein corresponding to the molecular mass of Abp1 on SDS-PAGE immunoblots of human Jurkat, Hela, and A431 cells, but does not detect Abp1 in mouse brain.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
image
New Releases
TP5691
image
TRPM7 (a.a. 1484-1497)
Rabbit Polyclonal
image
PM5991
image
PKCγ (C-terminus) M599
Mouse Monoclonal
image
LM5971
image
Laminin γ1 subunit (Domain IV) M597
Mouse Monoclonal
image