ECM Logo Bar
Search :
Technical Data Sheet

AM5581

image
image
image
ALDH1A1 (N-terminal region) M558
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms
55 kDa

image
image
Enlarge

Western blot image of human A431 (lane 1), HepG2 (lane 2), PC3 (lane 3), Jurkat (lane 4), bovine tubulin (lane 5), and recombinant human ALDH1A1 (lane 6). The blot was probed with mouse monoclonal ALDH1A1 M558 (lanes 1-6) at a dilution of 1:1,000.

Application
Dilution


ELISA
1:2000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
Aldehyde dehydrogenase (ALDH) superfamily is a ubiquitous group of enzymes found in all taxonomic domains. ALDH detoxifies endogenous and exogenous aldehydes, protecting cellular homeostasis and organismal functions. These enzymes are necessary for the synthesis of retinoic acid, betaine, and folate. Recent studies have reported high levels of ALDH found in cancer cells, suggesting that ALDH can act as a marker for cancer cells found in a wide variety of tissues including skin, prostate, lung, and neural tissues. Additionally, certain diseases can be identified when ALDH activity is absent. ALDH1A1 is vital for retinol synthesis and alcohol metabolism. ALDH1A1 active sites include an active cysteine residue, which catalyses the transformation of aldehydes into their respective carboxylic groups. ALDH1A1 amino acid sequence and function is highly conserved in humans and rodents.


Background References
Duester, G. (2000) Eur J Biochem. 267(14):4315.
Edenberg, H.J. (2007) Alcohol Res Health. 30(1):5.
Jackson, B. et al. (2011) Hum Genomics. 5(4):283.
Luo, Y. et al. (2012) Stem Cells. 30(10):2100.
Immunogen
Clone M558 was generated from a recombinant protein corresponding to amino acids in the N-terminal region from human ALDH1A1. This sequence is highly conserved in rat and mouse ALDH1A1.
Buffer and Storage
Mouse monoclonal purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The antibody was purified by Protein G chromatography. This antibody antibody detects a 55 kDa* protein on SDS-PAGE immunoblots of human A431, HepG2, and mouse liver, as well as human recombinant ALDH1A1. The antibody does not detect ALDH1A3 in PC3 cells, ALDH1A2 in Jurkat cells, and bovine tubulin (55 kDa).

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
AK6060
Actin & Tubulin Antibody Sampler Kit

AK7600
Actin Filament Regulation Immunocytochemistry Kit

AL9171
A431 Lysate

AM5621
ALDH1A1 (N-terminal region) M562 Mouse Monoclonal

MK6050
MAP Kinase Activation Antibody Sampler Kit

New Releases
IM0061
image
Integrin β1 (Extracellular region) M006
Mouse Monoclonal
image
TP5671
image
TRPM7 (Ser-1513), phospho-specific
Rabbit Polyclonal
image
CM5881
image
CD44 (Hyaluron Binding Region) M588
Mouse Monoclonal
image