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Technical Data Sheet

AM5771

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Adiponectin (C-terminal region) M577
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms
30 kDa

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Western blot analysis of mouse white adipose tissue (lanes 1 & 3) and human recombinant full length adiponectin (lanes 2 & 4). The blot was probed with mouse monoclonals anti-Adiponectin (C-terminal) antibody M577 (lanes 1 & 2) or anti-Adiponectin (C-terminal) antibody M576 (lanes 3 & 4).

Application
Dilution


ELISA
1:1000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Adiponectin is an adipokine that is secreted primarily from adipocytes and functions in glucose regulation and lipid metabolism. It has also been shown to play a major role in energy homeostasis. Studies report adiponectin secretion in bone, mammary glands, salivary glands, and cardiac tissue in limited quantities. It forms a homo-oligomeric structure and is homologous to complement factor C1q, collagen type VIII, and collagen type X. In humans and mice with low adiponectin levels, increased obesity and insulin resistance is observed. Adiponectin, typically an anti-inflammatory agent, has been shown to exert pro-inflammatory effects in nonmetabolic disease such as irritable bowel syndrome. Low levels of adiponectin in blood have been correlated with higher incidence and poorer prognosis in several cancers, including breast cancer.


Background References
Scherer, P.E. et al. (1995). J Biol Chem. 270(45):26746
Hu, E. et al. (1996). J Biol Chem. 271(18):10697
Waki, H. et al. (2003) J Biol Chem. 278(41):40352
Nalabolu, M. et al. (2014). Int J Hem Onc St Cell Res8(1):31
Immunogen
Clone (M577) was generated from a recombinant protein that included amino acid residues within the C-terminal region of mouse adiponectin. This sequence has high homology with similar regions in rat and human adiponectin.
Buffer and Storage
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol,
1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The antibody was purified using Protein G chromatography. This antibody detects a 30 kDa* protein on SDS-PAGE immunoblots of human and mouse serum, as well as detects full length human and mouse recombinant proteins.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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