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Technical Data Sheet

AP3851

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AIM2 (N-terminal region)
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
40 kDa

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Western blot analysis of human Jurkat cells (lane 1), mouse macrophages untreated (lane 2) and treated (lane 3) with IFNγ (10 ng/ml) and LPS (1µg/ml) for 12 hr (20 µg/lane). The blot was probed with rabbit polyclonal anti-AIM2 (N-terminal region) antibody at 1:1000.

Application
Dilution


ELISA
1:2000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Host- and pathogen-associated cytoplasmic double-stranded DNA triggers the activation of a NALP3-independent inflammasome, which activates caspase-1, leading to maturation of pro-interleukin-1beta and inflammation. Several studies have isolated AIM2 (absent in melanoma 2) as a candidate cytoplasmic-DNA-sensing protein that contains an N-terminal pyrin domain and C-terminal oligonucleotide binding domain. A screen for transcripts induced by interferon-beta identified AIM2 gene expression. AIM2 protein bound double-stranded DNA, recruited the inflammasome adaptor ASC, and localized to ASC containing speckles. AIM2 and ASC form a pyroptosome, which induces pyroptotic cell death mediated by caspase-1. RNA-mediated suppression of AIM2 expression impairs DNA-induced maturation of interleukin-1beta in THP-1 human monocytic cells, as well as abrogates caspase-1 activation in response to cytoplasmic double-stranded DNA and the double-stranded DNA vaccinia virus. Thus, AIM2 is a DNA-sensing protein for the activation of the caspase-1 inflammasome.


Background References
Bürckstümmer, T. et al. (2009). Nat Immunol. 10(3):266.
Fernandes-Alnemri, T. (2009) Nature. 458(7237):509.
Hornung, V. et al. (2009). Nature. 458(7237):514.
Roberts, T.L. et al. (2009). Science. 323(5917):1057.
Immunogen
AIM2 synthetic peptide (coupled to KLH) corresponds to amino acid residues in the N-terminal region of human AIM2. This peptide sequence is highly conserved in rat and mouse AIM2.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was affinity purified using AIM2 (N-terminal region) peptide (without carrier). The antibody detects a 40 kDa* doublet corresponding to AIM2 in immunoblots of Jurkat cells, as well as mouse macrophages treated with IFNγ and LPS.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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