ECM Logo Bar
Search :
Technical Data Sheet

AP4351

image
image
image
Arpc1b/p41-Arc (Thr-21), phospho-specific
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms, Ck, F
41 kDa

image
image
Enlarge

Western blot analysis of Arpc1b phosphorylation in human A431 stimulated with calyculin A (100 nM) for 30 min (lanes 1 & 3). The blots were treated with lambda phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Arpc1b (C-terminal region) (lanes 1 & 2) or anti-Arpc1b (Thr-21) (lanes 3 & 4).

Application
Dilution


ELISA
1:1000


WB
1:500



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Kim, Y.B. et al. (2015) J Biol Chem. 290(34):21076 WB: PC3 cells
Ho, H. et al. (2013) Pigment Cell Melanoma Res 26(2):218. WB: human A375 melanoma
image
Background
Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. p41-Arc (Arpc1b) subunit Arpc1 has two isoforms in humans, Arpc1a and Arpc1b. PAK1 can bind and phosphorylate Thr-21 in Arpc1b leading to growth factor-stimulated cell motility. In addition, Arpc1b colocalizes with γ-tubulin at centrosomes and stimulates Aurora A activity. Aurora A phosphorylates Arpc1b on Thr-21 and a nonphosphorylatable Arpc1b mutant cannot activate Aurora A kinase and centrosome amplification. Thus, Arpc1b has roles in cytoskeletal dynamics during cell motility and mitosis, and these activities are regulated by phosphorylation at Thr-21.


Background References
Welch, M.D. et al. (1997) J Cell Biol. 138(2):375.
Vadlamudi, R.K. et al. (2004) EMBO Rep. 5(2):154.
Molli, P.R. et al. (2010) J Cell Biol. 190(1):101.
Immunogen
Phospho-Arpc1b/p41-Arc (Thr-21) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding threonine 21 in human Arpc1b. This peptide sequence is highly conserved in rat, mouse, chicken, and fish Arpc1b, and has some homology to the conserved site in isoform Arpc1a.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was affinity purified using phospho-Arpc1b (Thr-21) peptide (without carrier). The antibody detects a 41 kDa* protein corresponding to the molecular mass of Arpc1b on SDS-PAGE immunoblots of human A431 and mouse C2C12 treated with calyculin A. This reactivity is removed with lambda phosphatase treatment.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
AK6060
Actin & Tubulin Antibody Sampler Kit

AK6390
Arp2/3 Complex Regulation Antibody Sampler Kit

AK7600
Actin Filament Regulation Immunocytochemistry Kit

AP4321
Arpc1b/p41-Arc (C-terminal region) Rabbit Polyclonal

CK6180
Coronin-1B Phospho-Regulation Antibody Sampler Kit

WK6110
N-WASP Phospho-Regulation Antibody Sampler Kit

New Releases
PM4991
image
PKC (α,β,γ) M499
Mouse Monoclonal
image
CM0331
image
CD55 (Extracellular region) M033
Mouse Monoclonal
image
CM5881
image
CD44 (Extracellular region) M588
Mouse Monoclonal
image