ECM Logo Bar
Search :
Technical Data Sheet

AP5291

image
image
image
Argonaute 2 (Ser-387), phospho-specific
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms, Ck, F
97 kDa

image
image
Enlarge

Western blot analysis of mouse recombinant Ago2 full length protein (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonal anti-Ago2 (AP5281) (lanes 1 & 2) and rabbit polyclonal anti-Ago2 (Ser-387) phospho-specific antibody (lanes 3 & 4).

Application
Dilution


ELISA
1:1000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
Several classes of small RNAs, including short interfering RNAs (siRNAs), microRNAs (miRNAs), and Piwi-interacting RNAs (piRNAs) have been identified. MicroRNAs are about 21 nucleotides in length and have been implicated in many cellular processes such as development, differentiation, and stress response. These small RNAs function together with complexes called micro-ribonucleoproteins (miRNPs) to regulate gene expression by modulating mRNA translation or stability. Among the most important components in these complexes are argonaute proteins. There are four members in the mammalian argonaute family and only argonaute 2 (Ago2) possesses the Slicer endonuclease activity. Argonaute proteins participate in various steps of microRNA-mediated gene silencing, such as repression of translation and mRNA turnover. These activities may be regulated by cell signaling events that alter argonaute phosphorylation. EGFR phosphorylates Tyr-393 in Ago2, which reduces binding to Dicer and inhibits miRNA processing. Akt3 phosphorylates Ago2 at Ser-387 leading to reduced mRNA cleavage and enhanced translational repression.


Background References
Peters, L. & Meister, G. (2007) Mol Cell 26: 611.
Filipowicz, W. et al. (2008) Nat Rev Genet. 9:102.
Horman, S.R. et al. (2013) Mol Cell 50:356.
Shen, J. et al. (2013) Nature 497:383.
Immunogen
Phospho-Ago2 (Ser-387) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 387 in human Ago2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2. The site is also conserved in Ago1 and Ago4, but the homology surrounding the site is low between Ago2 and these two family members.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was cross-absorbed to unphospho-Ago2 (Ser-387), before affinity purification using phospho-Ago2 (Ser-387) peptide (without carrier). The antibody detects a 97 kDa* protein corresponding to the apparent molecular mass of phosphorylated Ago2 on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A and mouse recombinant Ago2 full length protein. This reactivity is not observed after lambda phosphatase dephosphorylation.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
AK6340
Akt Phospho-Regulation Antibody Sampler Kit

AK6970
Argonaute 2 Phospho-Regulation Antibody Sampler Kit

AM5271
Argonaute 2 (N-terminus) Rat Monoclonal

AP5281
Argonaute 2 (a.a. 389-398) Rabbit Polyclonal

AP5311
Argonaute 2 (Tyr-393), phospho-specific Rabbit Polyclonal

EK6160
EGFR Phospho-Regulation Antibody Sampler Kit

New Releases
TP5691
image
TRPM7 (a.a. 1484-1497)
Rabbit Polyclonal
image
IM5951
image
Integrin α5 (Extracellular region) M595
Mouse Monoclonal
image
AM5771
image
Adiponectin (C-terminal region) M577
Mouse Monoclonal
image