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Technical Data Sheet

AP5571

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AIFM1 (C-terminal region)
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
66 kDa

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Enlarge

Western blot image of human jurkat cells untreated (lanes 1 & 3) or treated with calyculin A (100 nM, 30 min.) (lanes 2 and 4). The blot was probed with rabbit polyclonals anti-AIFM1 (C-terminal region) at 1:500 (lanes 1 & 2) and anti-AIFM1 (Ser-116) phospho-specific antibody at 1:1000 (lanes 3 & 4).

Application
Dilution


ELISA
1:1000


WB
1:500



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Apoptosis-inducing factor (AIFM1, AIF, PDCD8) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis. AIF is expressed as a 66 kDa precursor protein before being N-terminally cleaved to 62 kDa and localized to the mitochondrial intermembrane space. In response to apoptotic stimuli, AIFM1 is released from the mitochondrial intermembrane as a 57 kDa fragment that can translocate to the nucleus. Treatment of isolated nuclei with recombinant AIF leads to early apoptotic events, such as chromatin condensation and large-scale DNA fragmentation. Studies of AIF knockout mice have shown that the apoptotic activity of AIF is cell type and stimuli-dependent. Also noted was that AIF was required for embryoid body cavitation, representing the first wave of programmed cell death during embryonic morphogenesis. Structural analysis of AIF revealed two regions, an oxidoreductase activity and a DNA binding domain. AIF has dual functions, a pro-apoptotic activity in the nucleus via its DNA binding and an anti-apoptotic activity via the scavenging of free radicals through its oxidoreductase activity.


Background References
Daugas, E. et al. (2000) FEBS Lett. 476:118.
Lipton, S.A. and Bossy-Wetzel, E. (2002) Cell 111:147.
Delavallee, L. et al. (2011) IUBMB Life. 63(4):221.
Immunogen
AIFM1 (C-terminal region) synthetic peptide (coupled to carrier) corresponding to amino acids in the C-terminal half of human AIFM1. This sequence is identical in rat and mouse AIFM1, and has low homology to AIFM2 and AIFM3.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The antibody was purified by ion exchange chromatography. This antibody antibody detects a triplet at 66 and 62 kDa* protein on SDS-PAGE immunoblots of human Jurkat and K562 cells, as well as mouse C2C12 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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