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Technical Data Sheet

AX1005

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phospho-Akt (Thr-34)
Blocking Peptide
Price
Size

$55
50 μg

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Application
Dilution


Blocking
1:1000


ELISA
50 ng/well



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Akt (PKB, Rac kinase) is a 60kDa ser/thr kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4-bisphosphate, which induces membrane translocation of Akt coincident with its phosphorylation at Thr-308 and Ser-473. Upon activation, Akt associates with members of the PKC family of kinases, such as PKCδ and PKCζ. Ceramide-activated PKCζ leads to phosphorylation of Thr-34 within the pleckstrin homology domain of Akt. This phosphorylation inhibits PIP3 binding to Akt preventing activation of the kinase and may lead to cermide-induced cell death.


Background References
Jones, P.F. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4171-4175.
Marte, B. & Downward. J. (1997) TIBS. 22:355-358.
Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808.
Sequence
Phospho-Akt (Thr-34) synthetic peptide corresponds to amino acid residues around threonine 34 of human Akt. This sequence is highly conserved in rat and mouse Akt.
Buffer and Storage
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The peptide is specifically recognized by anti-Akt (Thr-34) phospho-specific antibody (AP1001) in ELISA, and is recommended for use in blocking AP1271 reactivity in Western blot and immunocytochemistry.
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Akt (Thr-34), phospho-specific Rabbit Polyclonal

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