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Technical Data Sheet

AX4585

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Arp3 (C-terminal region)
Blocking Peptide
Price
Size

$55
50 μg

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Enlarge

Immunocytochemical labeling of Arp3 in aldehyde-fixed and NP-40-permeabilized rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Arp3 (C-terminal region) (AP4581) in the absence (left) or presence (right) of blocking peptide (AX4585). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Application
Dilution


Blocking
1:1000


ELISA
50 ng/well



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. Arp2 has two phosphosites, Thr-237 and Thr-238, that are evolutionarily conserved, and are phosphorylated along with Tyr-202 in response to growth factor stimulation. These phosphorylation events may regulate binding to the pointed end of actin filaments, and alanine substitutions of these Arp2 phosphosites inhibit membrane protrusions. Thus, phosphorylation may be another mode of Arp2/3 complex regulation in addition to the activity of nucleation-promoting factors.


Background References
Kelleher, J.F. et al. (1995). J Cell Biol. 131(2):385.
LeClaire, L.L. et al (2008). J Cell Biol. 182(4):647.
Soderling, S.H. (2009). Sci Signal. 2(55):pe5 (Review).
Sequence
Arp3 (C-terminal region) synthetic peptide corresponding to amino acid residues in the C-terminal region of human Arp3. This peptide sequence is highly conserved in rat, mouse, chicken, fish, and yeast Arp3 proteins.
Buffer and Storage
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide.
Store at –20°C. Stable for 1 year.
Specificity
The peptide is specifically recognized by anti-Arp3 (C-terminal region) antibody (AP4581) in ELISA, and has been shown to block the reactivity of AP4581 during Western blot. In addition, the peptide is recommended for use in blocking AP4581 reactivity in immunocytochemistry.
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AP3871
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AP4581
Arp3 (C-terminal region) Rabbit Polyclonal

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