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Technical Data Sheet

CK6150

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γ-Catenin Phospho-Regulation
Antibody Sampler Kit
Price

$395

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
CP1081 Tsuneki, M. et al. (2014) Mol Cell Biol. 34(24): 4485 IF: mouse EOMA, brain endothelial cells
CP4021 Lu, Y. et al.(2014) Stem Cells Dev. 23(15):1755. IF: chicken embryonic fibroblasts
CP1081 Qi, F. et al. (2013) Am J Pathol. 183(5):1654. WB: human mesothelial
CP4021 Condello, S. et al. (2013) FASEB Journal 27(8): 3100. WB: ovarian cancer cells
CM1111 Cooksley-Decasper, S. et al. (2012) PLoS One 7(10):e47985. WB: human macrophages
CP1081 Krejci, P. et al. (2012) PLoS ONE. 7(4):e35826. WB: rat chondrosarcoma cells
CP2971 Cooksley-Decasper, S. et al. (2012) PLoS One 7(10):e47985. WB: human macrophages
CP1081 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs
CP2961 Beard, R.S. et al. (2011) Blood. 118(7):2007. ELISA: mouse microvascular ECs
CP2961 Funakoshi S et al (2010) Am J Phys Gast Liv Phys 299(5):1054 WB: human colon cancer cells
Kit Summary
The γ-Catenin phospho-regulation antibody sampler kit can be used to examine phosphorylation of γ-Catenin at Tyr-133, Tyr-480, Tyr-550 and Tyr-644. The kit also includes monoclonal and polyclonal antibodies to monitor total expression levels of γ-Catenin.
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Immunocytochemical labeling of phosphorylated γ-Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were co-labeled with mouse monoclonal γ-Catenin (CM1111) or rabbit polyclonal γ-Catenin (Tyr-550) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy2 or Cy3.

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Western blot analysis of A431 cells stimulated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then treated with akaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-γ-Catenin (CM1111), anti-β-Catenin (Tyr-489)/γ-Catenin (Tyr-480) (CP2961), or anti-β-Catenin (CM1181).

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Background
Plakoglobin (γ-catenin) is a catenin family member identified as a component of desmosomes. γ-Catenin has high homology to β-catenin and, like β-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. One molecule of α-catenin and at least one molecule of β-catenin and γ-catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of γ-catenin. Similar catenin-binding domains found in cadherins, suggest a common mechanism for γ-catenin localization to both adherens junctions and desmosomes. Phosphorylation of tyrosine residues (Tyr-133, Tyr-480, Tyr-550, Tyr-644) in γ-catenin can modify its interactions with other proteins. Phosphorylation of Tyr-644 decreases γ-catenin association with α-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate Tyr-550, which increases γ-catenin binding to α-catenin. Thus, tyrosine phosphorylation may be important for regulation of γ-catenin protein-protein interactions within desmosomal complexes.

Background References
Ozawa, M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:4246.
McCrea, P.D. et al. (1991) Science 254:1359.
Roura, S. et al. (1999) J Biol Chem. 274(51) :36734.
Miravet, S. et al. (2003) Mol. Cell. Biol. 23(20) :7391.
Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287.
Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225.
Rhee, J. et al. (2007) Nat. Cell Biol. 9(8):883.
Buffer and Storage
Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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