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Technical Data Sheet

CK6230

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δ1-Catenin Phospho-Regulation
Antibody Sampler Kit
Price

$395

Kit Summary
The δ1-Catenin phospho-regulation antibody sampler kit may be used to examine phosphorylation of δ1-Catenin at Tyr-96, Tyr-228, Tyr-280, Tyr-302, and Thr-916. The kit also includes a monoclonal antibody to monitor total expression levels of δ1-Catenin.
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Western blot analysis of δ1-Catenin phosphorylation in A431 cells stimulated with pervanadate (1 mM) for 30 min. (lanes 1,3,5,7,9). The blot was then treated with alkaline phosphatase (lanes 2,4,6,8,10). Blots were probed with anti-δ1-Catenin (a.a. 275-285) (lanes 1 & 2), anti-δ1-Catenin (Tyr-96) (lanes 3 & 4), anti-δ1-Catenin (Tyr-228) (lanes 5 & 6), anti-δ1-Catenin (Tyr-280) (lanes 7 & 8) or anti-δ1-Catenin (Tyr-302) (lanes 9 & 10).

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Immunocytochemical labeling of δ1-Catenin in control (Top row) or calyculin A-treated A431 cells (Bottom row). The cells were labeled with rabbit polyclonal δ1-Catenin (Thr-916) (Left) or mouse monoclonal δ1-Catenin (a.a. 275-285) (Middle). Demonstration of the overlapping labeling patterns is shown on the right. The antibodies were detected using goat anti-rabbit DyLight® 488 or goat anti-mouse-DyLight® 594.

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Background
Catenins are molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics wiith signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. δ1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by δ1-Catenin, which can modulate RhoA, Rac and cdc42 activity. δ1-Catenin is phosphorylated at multiple tyrosine, serine and threonine sites both in vitro and in vivo. High levels of δ1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in δ1-Catenin associated with the plasma membrane.

Background References
Mariner, D.J. et al. (2001) J. Biol. Chem. 276:28006.
Reynolds, A.B. & Roczniak-Ferguson, A. (2004) Oncogene 23:7947.
Fukumoto, Y. et al. (2008) Exp. Cell Res. 314:52.
Buffer and Storage
Rabbit polyclonal and mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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