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Technical Data Sheet

CK6360

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Caspase Family
Antibody Sampler Kit
Price

$395

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
Kit Summary
The Caspase family antibody sampler kit can be used to detect the full length and cleaved products of Caspase-3, Caspase-6, Caspase-7, and Caspase-8. The kit also includes secondary reagent for detection of these antibodies.
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Western blot analysis of Caspase expression in human Jurkat cells. The blots were probed with mouse monoclonal anti-Caspase-3 at 1:500 (lane 1) and 1:1000 (lane 2) or mouse monoclonal anti-Caspase-6 at 1:250 (lane 3) and 1:500 (lane 4).

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Enlarge

Western blot analysis of Caspase expression in human Jurkat cells. The blots were probed with mouse monoclonal anti-Caspase-7 at 1:500 (lane 1) and 1:1000 (lane 2) or mouse monoclonal anti-Caspase-8 at 1:250 (lane 3) and 1:500 (lane 4).

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Background
The caspases are a group of cysteine enzymes which cleave proteins in response to intrinsic and extrinsic pathways that cause apoptotic cell death. The caspases can be grouped into two subgroups based on their roles in apoptosis. Initiator caspases (caspases 2, 8, 9, and 10) are activated through the apoptosis-signaling pathways and activate the effector caspases (caspases 3, 6, and 7) which carry out apoptosis. Caspase cascades are initiated through assembly of multiprotein complexes that trigger activation of the initiator caspases, which are then released and are able to activate the downstream effector caspases.

Background References
Adams, J.M. & Cory S. (2002) Curr Opin Cell Biol. 14(6):715.
Shi Y. (2002) Mol Cell. 9(3):459.
Salvesen, G.S. & Riedl SJ. (2008) Adv Exp Med Biol. 615:13.
Buffer and Storage
Mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.
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