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Technical Data Sheet

CK7650

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δ1-Catenin Phospho-Regulation
Immunocytochemistry Kit
Price

$455

Kit Summary
The δ1-Catenin phospho-regulation kit can be used for immunocytochemical labeling of phosphorylation at Tyr-228, Tyr-280, and Tyr-302 in δ1-Catenin. The kit includes a monoclonal antibody to examine the pattern of expression of the total population of δ1-Catenin. In addition, secondary reagents conjugated to DyLight® 488 or DyLight® 594 are included for labeling primary antibodies for detection using green (493Ex/518Em) or red (593Ex/618Em) filter sets.
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Immunocytochemical labeling of phosphorylated δ1-Catenin in A431 control (top) or treated with pervanadate (bottom). The fixed cells were labeled with mouse monoclonal antibodies to δ1-Catenin (a.a. 275-285), δ1-Catenin (Tyr-228), δ1-Catenin (Tyr-280), and δ1-Catenin (Tyr-302). The antibodies were detected using Goat anti-Mouse secondary antibodies conjugated to DyLight® 594.

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Background
Catenins have emerged as molecular sensors that integrate cell-cell junctions and cytoskeletal dynamics with signaling pathways that control morphogenesis and cell to cell communication. δ1-Catenin (p120 catenin) is a catenin family member which contains an N-terminal coiled-coil domain, a regulatory domain containing multiple phosphorylation sites, and a central Armadillo repeat domain. δ1-Catenin regulates E-cadherin turnover, and has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by δ1-Catenin, which can modulate RhoA, Rac, and cdc42 activity. δ1-Catenin is phosphorylated at multiple tyrosine, serine, and threonine sites both in vitro and in vivo. High levels of δ1-Catenin phosphorylated at Tyr-228 are commonly seen in several carcinoma cell lines and after EGFR activation. Many other tyrosine sites are also phosphorylated in the N-terminal region, including Tyr-96, Tyr-112, Tyr-280, and Tyr-302. In addition, Thr-310 and Thr-916 are constituitively phosphorylated in many cell types, however this phosphorylation may occur only in δ1-Catenin associated with the plasma membrane.

Background References
Mariner, D.J. et al. (2001) J. Biol. Chem. 276:28006.
Reynolds, A.B. & Roczniak-Ferguson, A. (2004) Oncogene 23:7947.
Fukumoto, Y. et al. (2008) Exp. Cell Res. 314:52.
Buffer and Storage
Mouse monoclonal and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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