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Technical Data Sheet

CK7680

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γ-Catenin Phospho-Regulation
Immunocytochemistry Kit
Price

$355

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
CM1111 Cooksley-Decasper, S. et al. (2012) PLoS One 7(10):e47985. WB: human macrophages
Kit Summary
The γ-Catenin phospho-regulation kit can be used for immunocytochemical co-localization of γ-Catenin phosphorylation at Tyr-550 compared to the total expression of γ-Catenin. The kit includes rabbit polyclonal and mouse monoclonal antibodies, along with goat-anti-rabbit conjugated to DyLight® 594 and goat anti-mouse conjugated to DyLight® 488 for dual labeling experiments.
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Enlarge

Immunocytochemical labeling of phosphorylated γ-Catenin in control (Top) and pervanadate-treated (Bottom) A431 cells. The cells were labeled with mouse monoclonal γ-Catenin (CM1111) and rabbit polyclonal γ-Catenin (Tyr-550) antibodies, then the antibodies were detected using Goat anti-Mouse DyLight® 488 and Goat anti-Rabbit DyLight® 594. The overlay showing co-labeling between the two antibodies is shown to the right.

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Background
Plakoglobin (γ-Catenin) is a catenin family member identified as a component of desmosomes. γ-Catenin has high homology to β-catenin and, like β-catenin, it can associate with the cadherins, E-cadherin and N-cadherin. One molecule of α-catenin and at least one molecule of β-catenin and γ-Catenin simultaneously bind to a single cadherin molecule. A 19-amino acid sequence of desmoglein was found to be critical for binding of γ-Catenin. Similar catenin-binding domains found in cadherins suggest a common mechanism for γ-Catenin localization to both adherens junctions and desmosomes. Phosphorylation of tyrosine residues in γ-Catenin can modify its interactions with other proteins. Phosphorylation of tyrosine 644 decreases γ-Catenin association with α-catenin, but increases binding to desmoplakin. Fer kinase can phosphorylate tyrosine 550, which increases γ-Catenin binding to α-catenin. Thus, tyrosine phosphorylation may be important for regulation of γ-Catenin protein-protein interactions within desmosomal complexes.

Background References
McCrea, P.D. et al. (1991) Science 254:1359.
Miravet, S. et al. (2003) Mol. Cell. Biol. 23(20) :7391.
Buffer and Storage
Mouse monoclonal, rabbit polyclonal, and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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