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Technical Data Sheet


Cell Structure Labeling
Immunocytochemistry Kit


Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
PM1071 Omori, K. et al. (2015) Bioch Biophys Res Com 458(4): 934. IF: mouse NIH3T3 fibroblasts
PM1071 Cheng, I. et al. (2014) J Biol Chem. 289(39):26989 IF: B16-F1 cells
CM1181 Condello, S. et al. (2013) FASEB J 27(8):3100. WB & IHC: Ovarian Cancer Cells
CM1181 Yakubov, B. et al. (2013) Neoplasia 15(6):609. WB: Human Ovarian Cancer Cells
PM1071 Lee, S. et al. (2013) Biochim Biophys Acta. 1830(8):4017. WB: rat C6 and human U373 gliomas
PM1071 Liu, H. et al. (2013) BMC Cancer. 13:80. WB: human ovarian cancer cells
TM4111 Gehrig, S.M. et al. (2012) Nature. 484(7394):394. WB: mouse skeletal muscle
Kit Summary
The cell structure labeling kit can be used to identify important cell structures found in eukaryotic cells. The kit includes a panel of antibodies that detect the following cell structures:

Actin Filaments = Phalloidin:FITC
Cell Junctions = β-catenin
Focal Adhesions= Paxillin
Intermediate Filaments = Vimentin
Microtubules = α-Tubulin
Nuclear Envelope = Nucleoporin p62

Immunocytochemical labeling of nucleoporin p62 (NM4361; top row left) paxillin (PM1071; top middle), β-Catenin (CM11811; top right) vimentin (VM4341; bottom left), Phalloidin:FITC (PF7501; bottom middle), α-Tubulin (TM4111; bottom right). The antibodies were detected using either Goat anti-Mouse conjugated to DyLight® 594 or DyLight® 488.

Some of the major cell structures that form eukaryotic cells include a plasma membrane, cytoskeleton, nuclear envelope, cell junctional complexes and extracellular adhesion sites. The cytoskeleton includes actin filaments, microtubules, and intermediate filaments. These major cytoskeletal filaments are important for cell motility, cell adhesion, and cell morphology. Focal adhesions are protein complexes localized to the inner side of the plasma membrane at sites of adhesion. These complexes are important for adhesion to other cells and to the extracellular matrix. Paxillin is an important component of focal adhesions, while β-catenin is an important part of cell-cell junctions. The nucleus is formed from intermediate filament proteins that form a nuclear envelope. Nucleoporin p62 is an important protein in the nuclear pores that facilitate protein transport across the nuclear envelope.

Background References
Wulf, E. et al. (1979) Proc. Nat. Acad. of Sci.(USA). 76:4498.
Faulstich, H. et al. (1983) Exp. Cell Res. 144:73.
Faulstich, H. et al. (1988) J. Muscle Res. Cell Motility. 9:370.
Diaz-Nido, J. et al. (1990) J Biol. Chem. 265(23):13949.
Ozawa, M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:4246.
Carmo-Fonseca M. et al. (1991) Eur J Cell Biol. 55(1):17-30.
Newmeyer, D.D. (1993) Curr Opin Cell Biol. 5(3):395.
Sin, W.C. et al (1998) Mol Cell Biol. 18(11):6325.
Goto, H. et al. (1998) J Biol Chem. 273(19):11728.
Roura, S. et al. (1999) J Biol Chem. 274(51) :36734.
Westermann, S. & Weber, K. (2003) Nat. Rev. Mol. Cell. Biol. 4:938.
Piedra, J. et al. (2003) Mol. Cell. Biol. 23(7):2287.
Woodrow, M.A. (2003) Exp. Cell. Res. 287(2):325-338.
Huang, C. et al. (2003) Nature 424:219-223.
Huang, C. et al. (2004) Cell Cycle 3(1):4-6.
Brembeck, F.H. et al. (2004) Genes Dev. 18(18):2225.
Fourest-Lieuvin, A. et al. (2006) Mol. Biol. Cell. 17(3):1041.
Hermann, H. et al. (2009) J Clin. Invest. 119(7):1772.
Abeyweera, T.P. et al. (2009) J Biol Chem. 284(26):17648.
Buffer and Storage
Mouse monoclonal and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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