ECM Logo Bar
Search :
Technical Data Sheet

CL7321

image
image
image
Cofilin 1 + LIMK1
Lysate
Price
Size

$75
100 μl

image
Application
Dilution


WB
10 μl/lane



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell migration, cytokinesis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major amino acid differences found in the C-terminal region. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo.

For preparation of Ser-3 phosphorylated Cofilin, purified human Cofilin 1 was phosphorylated in vitro with His-tagged mouse recombinant LIM Kinase 1 for 30 min. at 37°C (Cat. #CL7321). For control lysate, the recombinant Cofilin 1 protein was exposed to buffer only (Cat.#CL7311). The phosphorylated product is detected by anti-Cofilin (Ser-3; Cat.#CP1151), while both the control and phosphorylated recombinant cofilin 1 are detected by anti-Cofilin (N-terminus; Cat.#CP1131).

Buffer and Storage
Cofilin 1 Control Lysate is supplied at a concentration of 10 ng/µl in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
imageRelated Products
CL7311
Cofilin 1 Control Lysate

CP1131
Cofilin 1 (N-terminus) Rabbit Polyclonal

CP1151
Cofilin 1 (Ser-3), phospho-specific Rabbit Polyclonal

LP1831
LIMK1 (C-terminus) Rabbit Polyclonal

LP1891
LIMK1 (Thr-508)[LIMK2 (Thr-505], phospho-specific Rabbit Polyclonal

New Releases
PM5991
image
PKCγ (C-terminus) M599
Mouse Monoclonal
image
CM0071
image
CD44 (Extracellular region) M007
Mouse Monoclonal
image
CM0331
image
CD55 (Extracellular region) M033
Mouse Monoclonal
image