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Technical Data Sheet

CM5881

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CD44 (Extracellular region) M588
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu
80 kDa

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Western blot analysis of dentaured (1 & 3) and native (lanes 2 & 4) human MDA-MB-231 whole cell lysates. The blots were probed with mouse monoclonal anti-CD44 (CM5881) at 1:1000 (lanes 1 & 2) or mouse monoclonal anti-CD44 (CM5911) at 1:1000 (lanes 3 & 4).

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Immunocytochemical labeling of CD44 in paraformaldehyde fixed human MDA-MB-231 cells. The cells were labeled with mouse monoclonal anti-CD44 (M588). The antibody was detected using goat anti-mouse DyLight® 594.

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Application
Dilution


ELISA
1:2000


ICC
1:100


IP
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Cell surface adhesion protein CD44 is a ubiquitously expressed type I transmembrane protein that has important functions related to cell-cell adhesion and extracellular matrix interactions. The transmembrane protein is post-translationally modified at multiple sites by glycosylation and phosphorylation. CD44 ligands include hyaluronic acid, collagens, laminins, osteopontin, serglycin, and fibronectin. CD44 has been implicated in inflammatory cell functions, as well as in tumor growth and metastasis. CD44 is overexpressed in many types of cancer; the interaction between CD44 and HER2 has been linked to an increase in ovarian carcinoma cell growth. CD44 interacts with ezrin, radixin, and moesin to link the actin cytoskeleton to the plasma membrane and the extracellular matrix. These interactions are critical for CD44 function in cell-cell adhesion and cell motility.


Background References
Tsukita, S., et al. J Cell Biol. 1994. 126(2):391
Goodinson, S., et al. J Clin Path: Mol Path 1999. 52(4):189
Cichy, J., et al. J Cell Biol. 2003. 161(5):839
Immunogen
Clone M588 was generated from a proprietary antigen related to the extracellular region of human CD44.
Buffer and Storage
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
Clone M588 was purified using Protein G chromatography. The antibody detects 80-130 kDa* bands corresponding to the molecular mass of CD44 on SDS-PAGE immunoblots of "native" MDA-MB-231 cell lysates. The antibody also detects "native" recombinant human CD44 extracellular region. The antibody does not bind CD44 in denatured MDA-MB-231 cell lysates or after β-mercaptoethanol treatment of recombinant CD44 protein.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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