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Technical Data Sheet

CM5961

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P-Cadherin (N-terminal region) M596
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
120 kDa

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Enlarge

Western blot image of human A431 cells (lanes 1-4). The blots were probed with mouse monoclonals anti-P-Cadherin at 1:250 (lane 1) and 1:500 (lane 2), 1:1000 (lane 3) and 1:2000 (lane 4).

Application
Dilution


ELISA
1:1000


ICC
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. P-Cadherin (Cadherin-3) is localized in placenta while E-Cadherin (Cadherin-1) and N-Cadherin (Cadherin-2) are found in epithelial and neural tissues, respectively. P-Cadherin is expressed in normal epithelial cells and some cancer cells, and its sequence contains 5 cadherin domains in the extracellular region.


Background References
Qi, J. et al. (2006) Mol. Biol. Cell 17(3):1261.
Immunogen
Clone (M596) was generated from a recombinant protein containing amino acids in the N-terminal region of human P-Cadherin. This sequence is highly conserved in mouse and rat P-cadherin.
Buffer and Storage
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
Clone M596 was purified using Protein A chromatography. The P-cadherin antibody detects a 120 kDa* protein corresponding to endogenous P-Cadherin expressed in human A431 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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