ECM Logo Bar
Search :
Technical Data Sheet

CP2231

image
image
image
VE-Cadherin (a.a.770-781)
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
140 kDa

image
image
Enlarge

Western blot image of human umbilical vein endothelial cells. The blots were probed with rabbit polyclonal anti-VE-cadherin (a.a. 770-781) at 1:1000 (lane 1) and 1:4000 (lane 2). In addition, the antibody was used in the presence (lane 4) or absence (lane 3) of blocking peptide.

image
Enlarge

Immunocytochemical labeling of VE-Cadherin in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with rabbit polyclonal VE-Cadherin (a.a. 770-781), then the antibody was detected using appropriate secondary antibody conjugated to Cy3. Phase image (left) and fluorescent image (right).

image
Application
Dilution


ELISA
1:2000


ICC
1:200


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Bowers, S.L. et al. (2010) 1188:143. FB: negative control in Cell-Cell contact assay
image
Background
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. VE-cadherin (Cadherin 5) is the major cadherin found in endothelial cells and has important roles during angiogenesis and maintenance of barrier permeability. The cytoplasmic domain of VE-cadherin comprises the juxtamembrane domain that binds to the p120 catenin, and the carboxylterminal domain that interacts with β- or γ-catenins. Modulation of tyrosine phosphorylation on one or more of the nine tyrosine sites in the cytoplasmic domain may be important for regulating both angiogenesis and permeability. Phosphorylation of Tyr-658 and Tyr-731 alters catenin binding, restores cell migration, and decreases barrier permeability. While VEGF-induced phosphorylation of Tyr-685 occurs through c-Src, and regulates endothelial cell migration, but not permeability.


Background References
Baumeister U. et al. (2005) EMBOJ 24:1686.
Potter M.D. et al. (2005) J Biol. Chem. 280(36):31906.
Wallez Y. et al. (2007) Oncogene 26:1067.
Immunogen
VE-Cadherin synthetic peptide (coupled to carrier protein) corresponds to amino acids 770 to 781 in human VE-cadherin. This sequence has significant homology to the conserved site in rat and mouse, and has less than 50% homology with other cadherins.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was affinity purified with VE-cadherin (a.a. 770-781) peptide. The purified antibody detects a 140 kDa* band corresponding to VE-cadherin in western blots of human endothelial cells, and this reactivity can be specifically blocked using VE-cadherin peptide (CX2235).

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
CM1701
N-Cadherin (Cytoplasmic) Mouse Monoclonal

CP1801
N-Cadherin (Tyr-820), phospho-specific Rabbit Polyclonal

CP1921
E-Cadherin (a.a. 774-786) Rabbit Polyclonal

CP1951
N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Rabbit Polyclonal

CP1981
VE-Cadherin (Tyr-685), phospho-specific Rabbit Polyclonal

CX2235
VE-Cadherin (a.a.770-781) Blocking Peptide

New Releases
IK6700
image
Integrin β family
Antibody Sampler Kit
image
CM5911
image
CD44 (Hyaluron Binding Region)
Mouse Monoclonal
image
AM0081
image
β-Actin
Mouse Monoclonal
image