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Technical Data Sheet

CP3451

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α1-Catenin (Tyr-148), phospho-specific
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms, Ck
102 kDa

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Western blot analysis of rat PC12 cells treated with pervanadate (1 mM) for 30 min (lanes 1, 3, & 5) then the blot was treated with alkaline phosphatase (lanes 2, 4, & 6). The blot was probed with anti-α-Catenin monoclonal (lanes 1 & 2), anti-α1-Catenin (Tyr-148) phospho-specific (lanes 3 & 4), or anti-α1-Catenin (a.a. 143-153) (lanes 5 & 6).

Application
Dilution


ELISA
1:2000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
α-catenins are cadherin interacting proteins with homology to vinculin. Three α-catenin genes have been described including α1-catenin (αE-Catenin), α2-catenin (αN-catenin), and α3-catenin (αT-catenin). α1-catenin has 81% homology with α2-catenin and 60% homology with α3-catenin. These α-catenin isoforms may have similar roles since each binds cadherins. However, their expression patterns are both overlapping and distinct. α1-catenin was identified in epithelial cells, and is expressed in various cell types. α2-catenin is enriched in the nervous system, and α3-catenin is expressed highest in testis and heart. Phosphorylation may regulate the activity of α1-catenin, since tyrosine phosphorylation of Tyr-148 occurs during intercellular adhesion. This site is dephosphorylated by SHP2, which inhibits α1-catenin binding to β-catenin and translocation to the plasma membrane. Phosphorylation of α1-catenin at Tyr-148 may be important for inhibition of cell transformation, and dephosphorylation of this site may be important during SHP2-mediated cell transformation.


Background References
Herrenknecht, K. et al. (1991) Proc Natl Acad Sci U S A. 88(20):9156.
Hirano, S. et al. (1992) Cell. 70(2):293.
Janssens, B. et al. (2001) J Cell Sci. 114(17):3177.
Burks, J. & Agazie, Y.M. (2006) Oncogene 25:7166.
Immunogen
Phospho-α1-Catenin (Tyr-148) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 148 in human α1-Catenin. This peptide sequence is highly conserved in rat and mouse α1-Catenin, but is not conserved in α2-Catenin or α3-Catenin.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody was cross-adsorbed to unrelated phospho-tyrosine peptide before affinity purification using phospho-α1-Catenin (Tyr-148) peptide (without carrier). The antibody detects a 102 kDa* protein corresponding to the molecular mass of α1-Catenin on SDS-PAGE immunoblots of rat PC12 cells treated with pervanadate.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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