ECM Logo Bar
Search :
Technical Data Sheet

DM5931

image
image
image
Desmoglein-1 (C-terminal region) M593
Mouse Monoclonal IgG2b
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms, Ck
165 kDa

image
image
Enlarge

Western blot analysis of human A431 (lane 1) and human MDA-MB-231 (lane 2) cell lysates. The blots were probed with mouse monoclonal anti-Desmoglein-1 (DM5931) at a dilution of 1:1000.

Application
Dilution


ELISA
1:2000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
The Desmoglein family of transmembrane glycoproteins include desmoglein 1, 2, and 3. Desmoglein-1 (Dsg1, CDHF4) is found in specialized plasma membrane junctions known as desmosome junctions. These junctions serve as membrane anchorage domains for intermediate filaments and microfilaments. Actin microfilaments anchor at adherens junctions to form an array of junction sizes, shapes, and positions. These junctions generally contain E- or N-Cadherins whose cytoplasmic regions are associated with cytoplasmic proteins such as α- and β- catenins, vinculin, α-actinin, and radixin. Intermediate filaments (IFs) are anchored at desmosomes by the desmosome-specific cadherin family members, desmocollin and desmoglein. These proteins associate with cytoplasmic proteins such as desmoplakin and plakoglobin (γ-Catenin). Plakoglobin is the only junctional protein found at both desmosomes and adherens junctions and, therefore, plays a crucial role in cell adhesion.


Background References
Wheeler, GN, et al. (1991) PNAS. 88(11):4796.
Mathur, M, et al. (1994) J Biol Chem. 269(19):14075.
Immunogen
Clone M593 was generated from a recombinant protein containing amino acid residues in the c-terminal region of human desmoglein 1. This sequence has high homology with similar regions in rat and mouse desmoglein 1.
Buffer and Storage
Mouse monoclonal purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
Clone M593 was purified using Protein A chromatography. The antibody detects a 165 kDa* protein corresponding to the molecular mass of Desmoglein 1 on SDS-PAGE immunoblots of human A431, HeLa, and MDA-MB-231 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
CK6120
β-Catenin Phospho-Regulation Antibody Sampler Kit

CK6150
γ-Catenin Phospho-Regulation Antibody Sampler Kit

CK6260
E-Cadherin Phospho-Regulation Antibody Sampler Kit

CK6310
N-Cadherin Phospho-Regulation Antibody Sampler Kit

CM1111
γ-Catenin (C-terminal) Mouse Monoclonal

New Releases
IM5841
image
Integrin αV (Extracellular region) M584
Mouse Monoclonal
image
TP5681
image
TRPM7 (Ser-1569), phospho-specific
Rabbit Polyclonal
image
CM0011
image
CD98 (Extracellular region) M001
Mouse Monoclonal
image