ECM Logo Bar
Search :
Technical Data Sheet

IK6270

image
image
image
Integrin β4 Phospho-Regulation
Antibody Sampler Kit
Price

$395

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
IP1281 Coleman, D.T. et al. (2015) PLoS One. 10(5):e0125399 WB: HCC-1806, MCF-10A
IP1281 Soung, Y.H. et al. (2013) BMC Cell Biol. 1:14. WB: MDA-MB-231, MDA-MB-435
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
IP1281 Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
IP1281 Soung, Y. & Chung, J. (2011) Mol Cancer Ther. 10(5):883. WB: human A431, MDA-MB-231
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
IP1281 Yang, X. et al. (2010) Mol Cell Biol. 30(22):5306. WB: Y1494F mutants
IP1281 Kim, H.I. et al. (2008) Cancer Prev Res. 1(5):385. WB: human b4 transfectants
Kit Summary
The Integrin β4 phospho-regulation antibody sampler kit can be used to detect phosphorylation of Integrin β4 at Tyr-1494 and Tyr-1526. The kit also includes an antibody to examine total Integrin β4 expression levels and secondary reagents for rabbit polyclonal and mouse monoclonal antibody detection.
image
image
Enlarge

Western blot analysis of A431 cells serum starved overnight (lanes 1, 3, & 5) and treated with pervanadate (1 mM) for 30 min (lanes 2, 4, & 6). The blots were probed with rabbit polyclonal anti-integrin-β4 (Tyr-1526) (lanes 1 & 2) and anti-integrin-β4 (Tyr-1494) (lanes 3 & 4) or with mouse monoclonal anti-integrin-β4 (lanes 5 & 6).

image
Enlarge

Immunocytochemical labeling of integrin β4 in control (Top) and pervanadate-treated A431 cells (Bottom). The cells were labeled with mouse monoclonal anti-integrin β4 (Cytoplasmic region) (left) or rabbit polyclonals anti-integrin β4 (Tyr-1494) (middle) or anti-integrin β4 (Tyr-1526) (right), then the antibodies were detected using appropriate secondary antibodies conjugated to DyLight® 594.

image
Background
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin α6β4 receptors are found in basement membrane along with laminin-5. These receptors are expressed in epithelial, schwann, endothelial and some immune cells. The cytoplasmic domain of the integrin-β4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly, this site is critical for integrin α6β4 increases in carcinoma invasion.

Background References
Dans, M. et al. (2001). J Biol Chem. 276(2):1494-1502.
Shaw, L.M. (2001). Mol Cell Biol. 21(15):5082-5093.
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Buffer and Storage
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.
imageRelated Products
CK7720
Cytoskeletal Filament Labeling Immunocytochemistry Kit

IK6270
Integrin β4 Phospho-Regulation Antibody Sampler Kit

IX1285
phospho-Integrin β4 (Tyr-1494) Blocking Peptide

IX1295
phospho-Integrin β4 (Tyr-1526) Blocking Peptide

AK6060
Actin & Tubulin Antibody Sampler Kit

MS3011
Anti-Mouse Ig:DyLight® 488 Goat Polyclonal

MS3031
Anti-Mouse Ig:DyLight® 594 Goat Polyclonal

RS3201
Anti-Rabbit Ig:HRP Goat Polyclonal

RS3261
Anti-Rabbit Ig:DyLight® 488 Goat Polyclonal

RS3271
Anti-Rabbit Ig:DyLight® 594 Goat Polyclonal

RS3241
Anti-Rabbit Ig Light-Chain Specific, unconjugated Mouse Monoclonal

New Releases
TP5661
image
TRPM7 (Ser-1493), phospho-specific
Rabbit Polyclonal
image
CM5911
image
CD44 (Extracellular region) M591
Mouse Monoclonal
image
LM5971
image
Laminin γ1 subunit (Domain IV) M597
Mouse Monoclonal
image