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Technical Data Sheet

IK6750

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Integrin α family
Antibody Sampler Kit
Price

$405

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Kit Summary
The Integrin α family antibody sampler kit can be used to detect the expression level of Integrin α2, Integrin α5, Integrin αL, and Integrin α5. The kit includes high affinity mouse monoclonal antibodies to examine Integrin α expression levels in western blot, immunoprecipitation, and immunocytochemistry.
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Western blot analysis of human Jurkat whole cell lysate. The blot was probed with mouse monoclonal anti-integrin-αL (IM5941) at a dilution of 1:500 (lane 1), 1:1000 (lane 2), 1:2000 (lane 3) and 1:4000 (lane 4).

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Western blot analysis of human MDA-MB-231 (lane 1) and A431 (lane 2) cells. The blot was probed with mouse monoclonal anti-Integrin-α2 (IM5831) at 1:1000 dilution.

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Background
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. Integrin α5 and integrin β1 form a receptor for fibronectin and fibrinogen. This receptor is important for vascular development. Both integrin subunits of the fibronectin receptor are heavily glycosylated in their extracellular domains. This modification is essential for proper cell attachment to basal membranes. The integrin β3 subunit associates with integrin α2 in platelets where this glycoprotein complex acts as a fibrinogen receptor and mediates platelet aggregation. Integrin α5 has been reported to suppress apoptosis by a Bcl-2 pathway and the C-terminal region is critical for cell motility and cytoskeletal rearrangements. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members, while integrin β3 complexes with the integrin αV subunit to form the vitronectin receptor in endothelial cells.

Background References
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Iwamoto, D, Calderwood, D.(2015) Curr Opin Cell Biol. 36:41.
Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Buffer and Storage
Mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.
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