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Technical Data Sheet

IM0061

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Integrin β1 (Extracellular region) M006
Mouse Monoclonal IgG2a
Price
Size
Species Reactivity
MW

$295
100 μl
Hu
150 kDa

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Western blot analysis of native (lanes 1-3) and denatured (lanes 4-6) cell lysates from human A431 (lane 1 & 4), A549 (lane 2 & 5), and MDA-MB-231 (lane 3 & 6). The blots were probed with mouse monoclonal anti-Integrin-β1 (IM0061) at 1:1000 dilution.

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Immunocytochemical labeling of Integrin β1 in paraformaldehyde fixed human A431 cells. The cells were labeled with mouse monoclonal anti-Integrin β1 (clone M006). The antibody was detected using goat anti-mouse DyLight® 594.

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Application
Dilution


ELISA
1:2000


ICC
1:100


IP
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The β1 subfamily includes 12 distinct integrin proteins that bind to different extracellular matrix molecules. Integrin β1 has been implicated in various activities including embryonic development, blood vessel, skin, bone, and muscle formation, as well as tumor metastasis and angiogenesis.


Background References
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Immunogen
Clone M006 was generated from a proprietary antigen related to the extracellular region of human integrin β1 in complex with integrin α.
Buffer and Storage
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
Clone M006 detects 120-150 kDa* bands corresponding to the molecular mass of Integrin β1 on SDS-PAGE immunoblots of native human A431, A549, MDA-MB-231, and LNCaP cell lysates. The antibody also detects native recombinant human Integrin α1/β1, α2/β1, and α3/β1 complexes, but does not detect recombinant human Integrin α6/β4 or αV/β3 complexes. The antibody does not detect the denatured form Integrin β1 in cells or recombinant proteins. Clone M006 can be used in western blot, immunocytochemical labeling, ELISA, and immunoprecipitation.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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