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Technical Data Sheet

IM5811

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Integrin β3 (Extracellular region) M581
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms
100 kDa

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Western blot analysis of human umbilical vein endothelial cells (HUVEC). The blots were probed with mouse monoclonal anti-integrin-β3 (IM5811) at 1:250 (lane 1) and 1:1000 (lane 2).

Application
Dilution


ELISA
1:1000


ICC
1:50


WB
1:500



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The integrin β3 subunit associates with integrin α2 in platelets where this glycoprotein complex acts as a fibrinogen receptor and mediates platelet aggregation. In endothelial cells, integrin β3 complexes with the integrin αV subunit to form the vitronectin receptor. This receptor mediates endothelial cell adhesion to vitronectin, fibrinogen, von Willebrand factor, thrombospondin, laminin, and fibronectin.


Background References
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Iwamoto, D, Calderwood, D.(2015) Curr Opin Cell Biol. 36:41.
Immunogen
Clone M581 was generated from a recombinant protein containing amino acid residues in the extracellular region of human Integrin β3. This sequence has high homology with rat and mouse Integrin β3, and has low homology to other integrin family members.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol,
1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody detects a 100kDa* protein corresponding to the molecular mass of Integrin β3 on SDS-PAGE immunoblots of human platelets and endothelial cells (HUVEC).

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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