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Technical Data Sheet

IM5831

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Integrin α2 (Extracellular region) M583
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms
150 kDa

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Western blot analysis of human MDA-MB-231 (lane 1) and A431 (lane 2) cells. The blot was probed with mouse monoclonal anti-Integrin-α2 (IM5831) at 1:1000 dilution.

Application
Dilution


ELISA
1:1000


WB
1:500



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The integrin β3 subunit associates with integrin α2 in platelets where this glycoprotein complex acts as a fibrinogen receptor and mediates platelet aggregation. In endothelial cells, integrin β3 complexes with the integrin αV subunit to form the vitronectin receptor. This receptor mediates endothelial cell adhesion to vitronectin, fibrinogen, von Willebrand factor, thrombospondin, laminin, and fibronectin.


Background References
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Iwamoto, D, Calderwood, D.(2015) Curr Opin Cell Biol. 36:41.
Immunogen
Clone M583 was generated from a recombinant protein containing amino acid residues in the extracellular region of human Integrin α2. This sequence has high homology with rat and mouse Integrin α2, and has low homology to other integrin family members.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol,
1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody detects a 150 kDa* protein corresponding to the molecular mass of Integrin α2 on SDS-PAGE immunoblots of human MDA-MB-231 and A431 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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