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Technical Data Sheet

IM5941

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Integrin αL (Extracellular region) M594
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
180 kDa

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Western blot analysis of human Jurkat whole cell lysate. The blot was probed with mouse monoclonal anti-integrin-αL (IM5941) at a dilution of 1:500 (lane 1), 1:1000 (lane 2), 1:2000 (lane 3) and 1:4000 (lane 4).

Application
Dilution


ELISA
1:1000


ICC
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. The integrin β2 subunit associates with integrin αL to form a receptor for ICAM family members. Integrin β2/αL is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes.


Background References
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Immunogen
Clone M594 was generated from a recombinant protein containing amino acid residues in the extracellular region of human Integrin αL. This sequence has low homology to other integrin family members.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol,
1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
Clone M594 was purified using Protein A chromatography. The antibody detects a 180 kDa* protein corresponding to the molecular mass of Integrin αL on SDS-PAGE immunoblots of human Jurkat cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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