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Technical Data Sheet

IM5951

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Integrin α5 (Extracellular region) M595
Mouse Monoclonal IgG2a
Price
Size
Species Reactivity
MW

$245
100 μl
Hu
150 kDa

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Western blot analysis of human HeLa cell lysates. The blot was probed with mouse monoclonal anti-Integrin-α5 (IM5951) at a dilution of 1:500 (lane 1) and 1:2000 (lane 2).

Application
Dilution


ELISA
1:2000


IP
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin receptors contain noncovalently associated α and β subunits that consist of a large extracellular region (the ligand-binding domain), a short transmembrane region, and a cytoplasmic domain of varying length. In mammals, at least 17 α subunits and 8 β subunits have been identified and these proteins can heterodimerize to form at least 22 different receptors. Integrin α5 and integrin β1 form a receptor for fibronectin and fibrinogen. This receptor is important for vascular development. Both integrin subunits of the fibronectin receptor are heavily glycosylated in their extracellular domains. This modification is essential for proper cell attachment to basal membranes. Integrin α5 has been reported to suppress apoptosis by a Bcl-2 pathway and the C-terminal region is critical for cell motility and cytoskeletal rearrangements.


Background References
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Iwamoto, D. & Calderwood, D. (2015) Cur Opin Cel Bio. 36:41.
Immunogen
Clone M595 was generated from a recombinant protein containing amino acid residues in the extracellular region of human Integrin α5. This sequence has low homology to other integrin family members.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol,
1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
Clone M595 was purified using Protein A chromatography. The antibody detects a 150 kDa* protein corresponding to the molecular mass of Integrin α5 on SDS-PAGE immunoblots of human HeLa and A431 cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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