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Technical Data Sheet

IX1295

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phospho-Integrin β4 (Tyr-1526)
Blocking Peptide
Price
Size

$55
50 μg

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Western blot analysis of A431 cells untreated(lanes 1, 5, & 9) or treated with pervanadate (1 mM) for 30 min (lanes 2-4, 6-8, & 10). The blots were probed with anti-Integrin β4 (Tyr-1494), anti-Integrin β4 (Tyr-1526), or anti-Integrin β4 (cytoplasmic) antibody. In some lanes, the antibodies were used in the absence (lanes 2 & 6) or presence of Integrin β4 (Tyr-1494) (lanes 3 & 7), or Integrin β4 (Tyr-1526) (lanes 4 & 8) blocking peptides.

Application
Dilution


Blocking
1:1000


ELISA
50 ng/well



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Integrins are cell adhesion molecules that can mediate bidirectional transfer of signals across the plasma membrane. The cytoplasmic domains of integrin family members interact with components of the signal transduction apparatus within cells. Integrin α6β4receptors are found in basement membrane along with laminin-5. These receptors are expressed in epithelial, schwann, endothelial and some immune cells. The cytoplasmic domain of the Integrin β4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly this site is critical for integrin α6β4 increases in carcinoma invasion.


Background References
Dans, M. et al. (2001). J Biol Chem. 276(2):1494-1502.
Shaw, L.M. (2001). Mol Cell Biol. 21(15):5082-5093.
Wang, L. et al. (2012) J Cell Physiol. 227(2):474.
Sequence
Phospho-Integrin β4 (Tyr-1526) synthetic peptide corresponds to amino acid residues around tyrosine 1526 of human Integrin β4. This peptide sequence is found in all three Integrin β4 isoforms and is highly conserved in rat and mouse Integrin β4.
Buffer and Storage
Blocking Peptide is supplied in 50µl phosphate-buffered saline and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The peptide is specifically recognized by anti-Integrin β4 (Tyr-1526) phospho-specific antibody (IP1291) in ELISA, and has been shown to block the reactivity of IP1291 during Western blot. In addition, the peptide is recommended for use in blocking IP1291 reactivity in immunocytochemistry.
imageRelated Products
IP1281
Integrin β4 (Tyr-1494), phospho-specific Rabbit Polyclonal

IP1291
Integrin β4 (Tyr-1526), phospho-specific Rabbit Polyclonal

IX1285
phospho-Integrin β4 (Tyr-1494) Blocking Peptide

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