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Technical Data Sheet

JK7630

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JNK Phospho-Regulation
Immunocytochemistry Kit
Price

$355

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Kit Summary
The JNK1 phospho-regulation kit may be used to examine the pattern of phosphorylation of JNK1 at the dual site (Thr-183/Tyr-185) relative to the total JNK1 pattern. The kit includes monoclonal antibodies to JNK1 (C-terminal region) and JNK1 (Thr-183/Tyr-185), as well as Goat anti-mouse secondary reagents conjugated to DyLight® 488 or DyLight® 594 for detection using green (493Ex/518Em) or red (593Ex/618Em) filter sets.
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Enlarge

Immunocytochemical labeling of JNK in control (left) or calyculin A-treated A431 cells (right). The cells were labeled with mouse monoclonal JNK (C-terminal region) (top) or mouse monoclonal JNK (Thr-183/Tyr-185) (bottom). The antibodies were detected using goat anti-mouse DyLight® 594 and DyLight® 488, respectively.

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Background
The stress-activated protein kinases (SAPK) or Jun-amino-terminal kinases (JNK) are potently activated by stressors such as UV and gamma radiation. Similar to other MAP Kinases, the core signaling unit is composed of a MAPKKK, usually MEKK1-4 or a mixed lineage kinase (MLK), which phosphorylate and activate MKK4-7, leading to dual phosphorylation and activation of JNK kinases. Rho-GTPases (Rac1 and cdc42) can stimulate MEKKs and MLKs, while MKKs can be activated by a GTPase-independent pathway that involves the germinal center kinase family. There are three JNK genes (JNK1,2,3) with further diversification resulting from alternative splicing. Active JNK dimers can translocate to the nucleus to regulate transcription through phosphorylation of c-Jun, ATF-2, and other transcription factors.

Background References
Whitmarsh, A.J. & Davis, R.J. (1998) Trends Biochem. Sci. 23:481.
Ichijo, H. (1999) Oncogene 18:6087.
Kyriakis, J.M. (1999) J. Biol. Chem. 274:5259.
Davis, R.J. (1999) Biochem. Soc. Symp. 64:1.
Buffer and Storage
Mouse monoclonal and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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