ECM Logo Bar
Search :
Technical Data Sheet

LM5971

image
image
image
Laminin γ1 subunit (Domain IV) M597
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms
220 kDa

image
image
Enlarge

Western blot image of human MDA-MB-231 cells (lanes 1-3). The blot was probed with mouse monoclonal anti-Laminin γ1 subunit (LM5971) at 1:500 (lane 1), 1:1000 (lane 2), and 1:2000 (lane 3).

Application
Dilution


ELISA
1:2000


ICC
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
The basal lamina contains Collagen Type IV, proteoglycans, and glycoproteins. Laminin is a high molecular weight (850 kDa) oligomer, consisting of three different chains (α, β, and γ) joined by disulfide bonds. The structure of laminins include two helical domains (I & II) at the COOH-terminal, a laminin IV domain, multiple EGF-like repeats, and a laminin globular domain (G), as well as an N-terminal domain VI. Domains IV and VI are the binding sites for collagen and heparan sulfate, respectively. Several isoforms have been identified for the genes of each chain. Laminin γ1 (laminin B2) contains 14 glycosylation sites and 12 cysteine repeat domains. The expression of the Laminin subunits is found in the basal lamina of tissues. Here, the protein interacts with other extracellular matrix components to mediate cell attachment, migration and organization during embryonic development.


Background References
Pikkarainen, T. et al. (1988) J Biol Chem. 263(14):6751.
Wewer, U.M. (1992) Lab Invest. 66(3):378.
Immunogen
Clone (M597) was generated from a recombinant protein containing amino acids in domain IV of human laminin γ1 subunit. This sequence is highly conserved in mouse and rat laminin γ1 subunits.
Buffer and Storage
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
Clone M597 was purified using Protein A chromatography. The laminin γ1 antibody detects a 220 kDa* protein corresponding to endogenous laminin γ1 expressed in human MDA-MB-231 and HeLa cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
IK6270
Integrin β4 Phospho-Regulation Antibody Sampler Kit

IM5811
Integrin β3 (Extracellular region) Mouse Monoclonal

IM5821
Integrin β1 (Extracellular region) Mouse Monoclonal

IM5831
Integrin α2 (Extracellular region) Mouse Monoclonal

IM5841
Integrin αV (Extracellular region) Mouse Monoclonal

New Releases
GK6980
image
Girdin Phospho-Regulation
Antibody Sampler Kit
image
IM5831
image
Integrin α2 (Extracellular region)
Mouse Monoclonal
image
IM5951
image
Integrin α5 (Extracellular region) M595
Mouse Monoclonal
image