ECM Logo Bar
Search :
Technical Data Sheet

MS3001

image
image
image
Anti-Mouse Ig:HRP
Donkey Polyclonal
Price
Size
Species Reactivity

$55
100 μl
Ms

image
image
Enlarge

Western blot analysis of rat brain probed with three different mouse monoclonal antibodies (Shown left to right in each panel: anti-PKC(α,β,γ), anti-paxillin, anti-nNOS). This panel of primary antibodies was then detected using varying dilutions of donkey anti-mouse Ig:HRP: Panel 1 = 1:1,250, Panel 2 = 1:2,500, Panel 3 = 1:5,000, Panel 4 = 1:10,000, Panel 5 = 1:20,000.

Application
Dilution


ELISA
1:10000


ICC
1:1000


IHC
1:1000


WB
1:5000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
image
Background
An array of chromogenic, fluorogenic and chemiluminescent substrates are available for use with HRP conjugated secondary antibodies HRP is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or the release of light as a byproduct of the reaction. HRP functions optimally at a near-neutral pH and can be inhibited by cyanides, sulfides and azides. Antibody-HRP conjugates are superior to antibody-AP conjugates with respect to the specific activities of both the enzyme and antibody. In addition, its high turnover rate, good stability, low cost and wide availability of substrates makes HRP the enzyme of choice for most applications. HRP can be used for chemiluminescent readout in western blot, colorimetric readout in ELISA, and precipitation reactions useful in immunocytochemistry and immunohistochemistry.


Background References
Mattson, D.L. & Bellehumeur, T.G. (1996). Anal. Biochem. 240:306.
Buffer and Storage
Donkey polyclonal antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, and 1 mg/ml BSA. Store at –20°C. Stable for 1 year. Note: Use of sodium azide as preservative substantially inhibits the enzyme activity of HRP.
imageRelated Products
RS3201
Anti-Rabbit Ig:HRP Goat Polyclonal

RS3251
Anti-Rabbit Ig Light-Chain Specific:HRP Mouse Monoclonal

RS3261
Anti-Rabbit Ig:DyLight® 488 Goat Polyclonal

RS3271
Anti-Rabbit Ig:DyLight® 594 Goat Polyclonal

New Releases
GP5791
image
Girdin (Ser-1416), phospho-specific
Rabbit Polyclonal
image
SK6710
image
Sox2 Thr-118/Lys-119 Regulation
Antibody Sampler Kit
image
IM5811
image
Integrin β3 (Extracellular region) M581
Mouse Monoclonal
image