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Technical Data Sheet

MS3011

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Anti-Mouse Ig:DyLight® 488
Goat Polyclonal
Price
Size
Species Reactivity

$125
0.5 ml
Ms

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Immunocytochemical labeling of β-Tubulin in A431 cells. The fixed cells were labeled with mouse monoclonal anti-β-Tubulin (TM1541) and the antibody was detected using Goat anti-Mouse secondary antibodies conjugated to DyLight® 488.

Application
Dilution


ICC
1:200


IHC
1:200



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
DyLight® fluorophores have absorption maxima covering the entire visible light spectrum (350 nm to 777 nm), as well several key near-infrared and infrared wavelengths. Both the absorption and emission properties of the DyLight® fluorophores match the excitation and detection wavelengths of common fluorescence instrumentation used for immunocytochemistry, immunohistochemistry, and flow cytometry. The DyLight® fluorophores exhibit higher fluorescence intensity and photostability than Alexa Fluor, CyDye and LI-COR Dyes in many applications and remain highly fluorescent over a broad pH range (pH 4-9). Additionally, the water solubility of the DyLight® fluorophores allows a high dye-to-protein ratio to be achieved without causing precipitation of conjugates. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make these fluorophores attractive as fluorescent probes in a variety of fluorescence methods.


Background References
Sarkar P. et al. (2010) J Photochem Photobiol B. 98(1):35.
Alkhatatbeh MJ (2011) J Thromb Haemost. 9(4):844.
Buffer and Storage
Goat polyclonal antibody is supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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