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Technical Data Sheet


Organelle Marker
Immunocytochemistry Kit


Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
GM3421 Martín-Acebes, M.A. et al. (2014) J virology 88(20): 12041. Data not shown
HP4291 Seo, Y. et al. (2013) PLoS ONE 8(8):e75005. WB: MDCK, A549, and HEK293
HP4291 Soe, K.C. et al. (2013) BBA 1829(11):1184. WB: Sf9 cells
GM3421 Martın-Acebes, M.A. et al. (2011) PLoS ONE 6(9): e24970. ICC: monkey Vero cell
Kit Summary
The organelle market kit can be used to co-localize proteins to specific organelles in cells. The kit includes a panel of antibodies that detect the following cell organelles:

Caveolae = Caveolin 1
Endoplasmic Reticulum = Calnexin
Endosomes = EEA1
Golgi = GM130
Mitochondria = Hsp60
Nucleus = Histone H2B

Immunocytochemical labeling of calnexin (CM4371) (top left), Hsp60 (HM4381) (top middle), EEA1 (EM3471) (top right), GM130 (GM3421) (bottom left), histone H2B (HP4291) (bottom middle), and caveolin-1 (CP2781) (bottom right). The antibodies were detected using secondary antibodies conjugated to DyLight® 488 or DyLight® 594.

A variety of subcellular compartments in eukaryotic cells perform specific activities related to cell function. The nucleus encloses DNA containing chromatin where gene transcriptions occurs. Endoplasmic reticulum, Golgi, and endosomes are involved in protein transport and protein post-translational modification and folding. Mitochondria contain proteins involved in cellular respiration, apoptosis, and free radical production. Caveolae are abundant cell-surface organelles involved in lipid regulation and endocytosis. Immunocytochemical co-labeling of newly discovered proteins with organelle marker antibodies is an important technique for determining protein function.

Background References
Venner, T.J. et al. (1990) DNA Cell Biol. 9(8):545.
Wada, I. et al. (1991) J Biol Chem. 266(29):19599.
Warren, G. (1993) Annual Rev Biochem 62:323.
Bergeron, J.J. et al. (1994) Trends Biochem Sci. 19(3):124.
Mu, F.T. et al. (1995) J. Biol. Chem. 270:13503.
Nakamura, N. et al. (1995) J Cell Biol 131:1715.
Workman, J.L. & Kingston, R.E. (1998) Annu Rev Biochem. 67:545.
Simonsen, A. et al. (1998) Nature 394:494.
Okamoto, T. et al. (1998) J. Biol. Chem. 273:5419.
Nomura, R. et al. (1999) Mol. Biol. Cell 10:975.
Smart, E. J. et al. (1999) Mol. Cell. Biol. 19:7289.
Weide, T. et al. (2001) EMBO Rep 2:336.
Dumas, J.J. et al. (2001) Mol. Cell 8:947.
Schlegel, A. et al. (2001) J. Biol. Chem. 276:4398.
Cappello, F. et al. (2008) Cancer Biol Ther. 7(6):801.
Ajiro, K. et al. (2010) Cell Death Differ. 17(6):984.
Bungard, D. et al. (2010) Science. 329(5996):1201.
Chevet, E. et al. (2010) Semin Cell Dev Biol. 21(5):486.
Grundtman, C. et al. (2011) Arterioscler Thromb Vasc Biol. 31(5):960.
Lau, A.T. et al. (2011) J Biol Chem. 286(30):26628.
Buffer and Storage
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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