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Technical Data Sheet

PK6330

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Phospho-Tyrosine, Serine, Threonine
Antibody Sampler Kit
Price

$395

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
PM3801 Fester, L. et al. (2015) JSBMB S0960-0760(15)30109. WB: Hippocampal neurons
PM3801 Osma-Garcia, I.C. et al. (2015) Eur J Immunol. doi: 10.1002 WB: mouse macrophages
PP2221 Hamada-Kawaguchi, N. et al. (2015) PLoS One. 10(3):e0121484 ICC: fly ovary
PP2551 Kommaddi, R.P. et al. (2015) J Biol Chem. 3;290(14):8888 WB: COS-7 cells
PP2551 Tsai, C.F. et al. (2015) J Agric Food Chem. 9;63(48):10388 WB: Huh7 cells
PM3801 Morinaga, J. et al. (2013) AJP Renal Phys. 305(2)F173. WB: Normal Rat Kidney Fibroblast
PP2221 Sroyraya, M. et al. (2013) Microsc Res Tech. 76(1):103. WB: Swimming Crab
PP2551 Kim, S. et al. (2013) Diabetes. 62(2):471. WB, IP: mouse adipocytes
PP2551 Sroyraya, M. et al. (2013) Microsc Res Tech. 76(1):103. WB: Swimming Crab
RS3251 Kawasaki, H. et al. (2013) World J Gastroenter. 19(17):2629. WB, ICC: mouse intestinal myofibroblasts
PP2551 Hummel, S. et al. (2012) Appl Environ Microbiol. 78(4):1140. WB: human colonic adenocarcinoma cells
MS3001 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
PP2551 Laluk, K. et al. (2011) Plant Cell 23(8):2831. WB: Arabidopsis BIK1 and Myelin basis protein
RS3251 Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
Kit Summary
The phospho-tyrosine, -serine, and -threonine antibody sampler kit can be used to detect changes in phosphorylation at tyrosine, serine, and threonine residues within proteins. The kit also includes secondary reagents for detection of these antibodies.
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Western blot analysis of A431 cells treated with calyculin A (100 nM) for 30 min (lane 1) then treated with lambda phosphatase (lane 2). The blot was probed with anti-Phosphoserine/threonine rabbit polyclonal at 1:1000.

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Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine (PP2221) and mouse monoclonal anti-Phosphotyrosine (PM3751), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

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Background
Phosphorylation of specific tyrosine (tyr), serine (ser), or threonine (thr) residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr and tyr kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine, phosphoserine, or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest, followed by detection of tyr, ser, and thr phosphorylation using these antibodies is commonly used to correlate changes in phosphorylation state with alterations in protein activity.

Background References
Ross, A.H. et al. (1981) Nature 294:654.
Hunter T.(1987) Cell. 50(6):823.
Wang, J.Y.J. (1988) Anal. Biochem 172:1.
Krishna, R.G. & Wold, F. (1993) Adv Enzymol Rel Areas Mol Biol 67:265.
Yaffe, M.B. & Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
Buffer and Storage
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide. Store all at –20°C. Stable for 1 year.
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