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Technical Data Sheet

PK7560

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PKC
Antibody Sampler Kit
Price

$455

Kit Summary
The PKC Antibody Sampler kit can be used to examine the expression levels of PKCα, PKCβ, PKCγ, PKCδ, and PKCθ. The kit includes mouse monoclonal antibodies that detect PKC (α,β,γ) isoforms (M499), as well as antibodies to detect the specific isoforms: PKCα, PKCβ, PKCγ, PKCδ, and PKCθ specifically.
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Western blot analysis of PKCβ in adult mouse brain lysate. The blot was probed with mouse monoclonal anti-PKCβ clone M598 at 1:1000 (lane 1) and 1:4000 (lane 2).

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Immunocytochemical labeling of PKCθ in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal PKCθ (N-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.

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Background
The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCα and PKCβI respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.

Background References
Nishizuka, Y. (1988) Nature 334:661.
Thiels, E. et al. (2000) J Neurosci. 20(19):7199.
Kawakami et al. (2003) PNAS. USA 100:9470-9475.
Buffer and Storage
Mouse monoclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.
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