ECM Logo Bar
Search :
Technical Data Sheet

PM2171

image
image
image
PKCθ (N-terminal region)
Mouse Monoclonal IgG2a
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
79 kDa

image
image
Enlarge

Western blot analysis of PKCθ in human Jurkat cell lysate. The blot was probed with anti-PKCθ at 1:250 (lane 1) and 1:1000 (lane 2).

image
Enlarge

Immunocytochemical labeling of PKCθ in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal PKCθ (N-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.

image
Application
Dilution


ELISA
1:2000


ICC
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. The phosphorylation of multiple sites in PKCs regulates their activity.


Background References
Nishizuka, Y. (1988) Nature 334:661.
Kawakami et al. (2003) PNAS. USA 100:9470-9475.
Immunogen
Clone (M217) was generated from a recombinant mouse PKCθ protein that included amino acids residues in the N-terminal region. This sequence is conserved in human and rat PKCθ, and has low homology to other PKC family members.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody detects a 79 kDa* protein corresponding to the molecular mass of PKCθ on SDS-PAGE immunoblots of human Jurkat cell lysates.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
PM1101
PKC (α,β2,γ) M110 Mouse Monoclonal

PM2371
PKCα (Central region) Mouse Monoclonal

PM2421
PKCδ (N-terminal region) Mouse Monoclonal

PP1091
PKCα (Ser-657/Tyr-658), phospho-specific Rabbit Polyclonal

New Releases
IM0061
image
Integrin β1 (Extracellular region) M006
Mouse Monoclonal
image
CM0011
image
CD98 (Extracellular region) M001
Mouse Monoclonal
image
IK6700
image
Integrin β family
Antibody Sampler Kit
image