ECM Logo Bar
Search :
Technical Data Sheet

PM5981

image
image
image
PKCβ (N-terminal region) M598
Mouse Monoclonal IgG2b
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms, Ck
82 kDa

image
image
Enlarge

Western blot analysis of PKCβ in adult mouse brain lysate. The blot was probed with mouse monoclonal anti-PKCβ clone M598 at 1:1000 (lane 1) and 1:4000 (lane 2).

Application
Dilution


ELISA
1:2000


ICC
1:50


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCα and PKCβI respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.


Background References
Nishizuka, Y. (1988) Nature 334:661.
Kawakami et al. (2003) PNAS. USA 100:9470-9475.
Immunogen
Clone (M598) was generated from a recombinant protein that included amino acids residues in the N-terminal region of human PKCβ.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody detects an 80 kDa* protein corresponding to the molecular mass of PKCβ on SDS-PAGE immunoblots of mouse brain lysates. The antibody detects full length human recombinant PKCβ1, but does not cross-react with human recombinant PKCα. The antibody also detects PKCβ in immunocytochemical assays.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
PK6540
PKC (α,β,γ) Phospho-Regulation Antibody Sampler Kit

PM1101
PKC (α,β2,γ) M110 Mouse Monoclonal

PM2371
PKCα (Central region) Mouse Monoclonal

PM2421
PKCδ (N-terminal region) Mouse Monoclonal

PM4991
PKC (α,β,γ) M499 Mouse Monoclonal

PM5991
PKCγ (C-terminus) M599 Mouse Monoclonal

New Releases
LM5971
image
Laminin γ1 subunit (Domain IV) M597
Mouse Monoclonal
image
GP5791
image
Girdin (Ser-1416), phospho-specific
Rabbit Polyclonal
image
AM5761
image
Adiponectin (C-terminal region) M576
Mouse Monoclonal
image