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Technical Data Sheet

PM5991

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PKCγ (C-terminus) M599
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms, Ck
82 kDa

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Western blot analysis of PKCγ in adult mouse brain lysate. The blot was probed with mouse monoclonal anti-PKCγ clone M599 at 1:500 (lane 1) and 1:2000 (lane 2).

Application
Dilution


ELISA
1:2000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester, while the C-terminal half contains the catalytic domain. The conventional PKC subfamily (α, β1, βII, and γ) is regulated by both Ca2+ and diacylglycerol. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters and growth factors. The phosphorylation of multiple sites in conventional PKCs regulates their activity. In mast cells, FceRI stimulation leads to phosphorylation of tyrosine 658 and 662 of PKCα and PKCβI respectively. This phosphorylation requires autophosphorylation of serine 657 and 661 in these respective kinases.


Background References
Nishizuka, Y. (1988) Nature 334:661.
Kawakami et al. (2003) PNAS. USA 100:9470-9475.
Immunogen
Clone (M599) was generated from a peptide corresponding to amino acids in the C-terminus of PKCγ. This sequence has low homology to all other PKC family members.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody detects an 80 kDa* protein corresponding to the molecular mass of PKCγ on SDS-PAGE immunoblots of mouse brain lysates. The antibody also detects a human recombinant PKCγ protein, but does not cross react with full length human recombinant PKCα or PKCβ.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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