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Technical Data Sheet

RM3351

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RasGAP
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms
120 kDa

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Enlarge

Western blot of mouse brain (lane 1), human A431 (lane 2), Jurkat (lane 3), and SYF fibroblast lysates. The blot was probed with mouse monoclonal anti-RasGAP antibody at 1:1000.

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Enlarge

Immunocytochemical labeling of RasGap in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal RasGap (RM3351) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.

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Application
Dilution


ELISA
1:2000


ICC
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
The Ras family (K-Ras, H-Ras, and N-Ras) includes 21 kDa guanine-nucleotide binding proteins that cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAP kinase pathway. Both GTPase-activating proteins (GAPs), such as p120 Ras-GAP, and guanine-nucleotide exchange factors (GEFs) regulate the activity of Ras proteins in many cell signaling pathways. Mutations in Ras are found in several cancers, and the most common oncogenic mutation is Gly12 to Asp (G12D), which prevents Ras inactivation.


Background References
Boguski, M.S. & McCormick, F. (1993) Nature 366:643.
Iwashita, S. & Song, S.Y. (2008) Mol Biosyst. 4(3):213.
Immunogen
Clone (M335) was generated from a recombinant sequence in the central region of human RasGAP. This sequence has high homology to mouse and rat RasGAP.
Buffer and Storage
Mouse monoclonal, protein A purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Specificity
This antibody detects a 120 kDa* protein corresponding to the molecular mass of RasGAP on SDS-PAGE immunoblots of adult mouse brain, human A431, Jurkat, and SYF fibroblasts.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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