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Technical Data Sheet

RS3241

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Anti-Rabbit Ig Light-Chain Specific, unconjugated
Mouse Monoclonal
Price
Size
Species Reactivity

$55
100 μl
Rb

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Application
Dilution


ELISA
20 ng/lane



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Estrada-Bernal, A. et al. (2011) J Neurooncol. 102:353. Western Blot
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Background
An array of antibody-based immunoassays require the use of secondary reagents that detect mouse, rabbit, rat, or goat primary antibodies. These secondary reagents are usually conjugated to a detection reagent, such as horse radish peroxidase (HRP). HRP is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or the release of light as a byproduct of the reaction. HRP functions optimally at a near-neutral pH and can be inhibited by cyanides, sulfides and azides. In addition, its high turnover rate, good stability, low cost and wide availability of substrates makes HRP the enzyme of choice for most applications. Mouse anti-Rabbit Ig Light-Chain Specific:HRP can be used for chemiluminescent readout in western blot, colorimetric readout in ELISA, and precipitation reactions useful in immunocytochemistry and immunohistochemistry. Mouse anti-Rabbit Ig Light-Chain Specific unconjugated is also available for ELISA sandwich assays and for antibody blocking experiments where no detection reagent is required.


Background References
Mattson, D.L. & Bellehumeur, T.G. (1996). Anal. Biochem. 240:306.
Buffer and Storage
Mouse monoclonal antibody purified with protein A chromatography is supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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