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Technical Data Sheet

SK7750

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Sema-3A & NRP1/Plexin A1 Receptor
Immunocytochemistry Kit
Price

$405

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
NP2111 Boro, A. et al. (2015) Am J Transl Res. 7(3):640-653 WB: human osteosarcoma cell lines
PP1301 Cheadle, L. et al. (2014) J of Neuroscience 34(23): 7999. WB: rat brain
NP2111 Ohkusu-Tsukada, K. et al. (2010) Int J Cancer. Epub 15 MAR. ELISA: sNRP1 mouse serum
NP2111 Drenberg, C.D. et al. (2009) Obstet Gynecol Intl. 2009:1-8. IHC: human ovarian tissues
SP1221 Pan, H. et al. (2009) Breast Can Res Treat. 118(1):197. WB: MDA-MB-231, shRNA knockdown
NP2111 Saban, M.R. et al. (2008) AJP Renal Phys. 295(1):F60. ICC: human urothelium
SP1221 Tang, X-Q. et al. (2007) J. Neurosci. 27(22):6068. WB: rat E14 spinal cord
Kit Summary
The Sema-3A & NRP1/Plexin A1 receptor kit can be used for immuncytochemical labeling of Sema-3A relative to its receptors, NRP1 and Plexin A1. The kit also includes secondary reagents conjugated to DyLight® 488 or DyLight® 594 for detection of primary antibodies using green (493Ex/518Em) or red (593Ex/618Em) filter sets.
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Immunocytochemical labeling of Sema-3A and its receptors in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-Sema-3A (C-terminal) (SP1221), anti-NRP1 (NP221), and anti-Plexin A1 (PP1301). The antibodies were detected using appropriate secondary antibody conjugated to either DyLight® 488 or DyLight® 594.

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Background
One family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI-anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co-receptors, Neuropilin-1 and Plexin-A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A-containing region. Thus, Sema3A and its co-receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.

Background References
Kolodkin, A.L. et al. (1993) Cell 75:1389.
Luo, Y. et al. (1993) Cell 75:217.
He, Z. & Tessier-Lavigne, M. (1997) Cell 90:739.
Tamagnone, L. et al. (1999) Cell 99:71.
Takahashi, T. et al. (1999) Cell 99:59.
Takahashi, T. & Strittmatter, S.M. (2001) Neuron 29:429.
Pasterkamp, R.J. & Verhaagen, J. (2001) Brain Res Rev 35:36.
Nakamura, F. & Goshima, Y. (2002) Adv Exp Med Biol. 515:55.
Buffer and Storage
Rabbit polyclonal antibodies and secondary reagents are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
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