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Technical Data Sheet

TM5711

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TRPM8 (Extracellular region) M571
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms
220 kDa

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Enlarge

Western blot image of human autophosphorylated TRPM8 in human MDA-MB-231 cells. The blot was probed with mouse monoclonal anti-TRPM8 (extracellular region) clone M571 (lane 1) or clone M572 (lane 2).

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Enlarge

Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-TRPM8 (M571). The antibody was detected using goat anti-mouse DyLight® 594.

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Application
Dilution


ELISA
1:2000


ICC
1:200


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
The Transient Receptor Potential Melastatin (TRPM) subfamily of cation-permeable channels is ubiquitous in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, some TRPM family members, TRPM6 and TRPM7, possess serine/threonine kinase activity and autophosphorylation. TRPM8 is thermoactivated at mildly cold temperatures (>25oC), and can also be activated by compounds that cause a cooling sensation, such as menthol and icilin. TRPM8 is expressed in trigeminal and dorsal root ganglia neurons where it confers sensitivity to cold in the somatosensory system. In vascular smooth muscle, TRPM8 may alter blood flow by constricting or enlarging blood vessels. TRPM8 is also expressed in normal prostate epithelial cells, as well as overexpressed in several primary tumors including colon, lung, skin, breast, and prostate cancers.


Background References
Asuthkar, S. et al. (2014) J Biol Chem. 290(5):2659
Potapova, T.A. et al. (2014) Bull Exp Biol Med. 157(6):757
Bibaux, G. et al. (2015) Proc Natl Acad Sci. 112(26):E3345
Shah, A.A. et al. (2015) Clin Exp Rheuma. 33(4S91):S123
Immunogen
TRPM8 synthetic peptide (coupled to carrier) corresponding to amino acids in the extracellular region of human TRPM8. This site is well conserved in rat and mouse TRPM8, but has low homology to other TRPM family members.
Buffer and Storage
Mouse monoclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The antibody was affinity purified using TRPM8 (Extracellular region) peptide. This antibody antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 and MCF-7 cells. The antibody is also recommended for immunocytochemical labeling of cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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