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Technical Data Sheet

TP5691

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TRPM7 (a.a. 1484-1497)
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$245
100 μl
Hu
220 kDa

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Enlarge

Western blot image of human autophosphorylated TRPM7 C-terminal kinase domain (lanes 1-6). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 phosphosites (lanes 2, 4, & 6). The blot was probed with rabbit polyclonals anti-TRPM7 (Ser-1513) phospho-specific (lanes 1 & 2), anti-TRPM7 (a.a.1484-1497) TP5691 antibody (lanes 3 & 4), or anti-TRPM7 (Ser-1493) phospho-specific (lanes 5 & 6).

Application
Dilution


ELISA
1:1000


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
The transient receptor potential melastatin (TRPM) subfamily of cation-permeable TRP channels is ubiquitously expressed in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, TRPM6 and TRPM7 possess an inherent serine/threonine kinase activity. TRPM7 specifically is involved with cellular magnesium homeostasis and neurotransmitter release. Due to the magnesium inhibition, TRPM7’s ion channel activity is very low. TRPM7 has been implicated in cell proliferation and migration during cancer progression, and its expression levels correlate with prognosis in breast cancer. TRPM7 kinase activation leads to massive autophosphorylation of the C-terminal region, including phosphorylation of Ser-1493, Ser-1513, and Ser-1569. Both Ser-1513 and Ser-1569 phosphorylation is required for kinase activity, and phosphorylation of Ser-1513 may inhibit Caspase-mediated cleavage of the C-terminal tail. Thus, TRPM7 is a multifunctional transmembrane protein with roles in cell signaling, proliferation, migration, and death.


Background References
Masayuki, M. et al. (2005) J of Bio Chem 280(21): 20793
Clark, K. et al. (2008) PLoS ONE 3(3): e1876
Desai, BN. et al. (2012) Dev. Cell. 22(6): 1149
Park, HS, et al. (2014) Korean J Phys Pharm. 18(1):15-23.
Immunogen
TRPM7 (a.a. 1484-1497) synthetic peptide (coupled to carrier) corresponding to amino acid 1484 to 1497 in human TRPM7. This site is not well conserved in rat and mouse TRPM7, and has no homology to other TRPM family members.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
The antibody was affinity purified using TRPM7 (a.a. 1484-1497) peptide. This antibody antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 cells and detects a 70 kDa recombinant C-terminal kinase domain of human TRPM7.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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